We narrowed to 2,970 results for: PPL

1. CMV::TVA/tRFP

   Plasmid

   #164486

   Purpose

   Dual CMV promoter plasmid to express TVA and TurboRFP

   Depositor

   Hollis Cline

   Article

   Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

   Inserts

   TVA (CD320 Chicken)
   TurboRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   sCMV and sCMV IE94

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS_N_3xFLAG

   Plasmid

   #101654

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, 3xFLAG tag
   

   Use

   Tags

   3xFLAG tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_m4_KRAB_KanR neo

   Plasmid

   #167877

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 35S::Cel1SP-GeNL/pCAMBIA1301

   Plasmid

   #190069

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Cel1 signal peptide-Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX SHIP2-SH2

   Plasmid

   #46493

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHIP2
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 8-131

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA KanR neo

   Plasmid

   #167909

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUM-Kan-MCS2

   Plasmid

   #109289

   Purpose

   Expresses high levels of protein in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   pSUM-kan-MCS1-gfp was modified to reorient the Ba…

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_KanR neo

   Plasmid

   #167869

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAX_CAGGS_CAS9_m4_VPR_Hygro

   Plasmid

   #167883

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSPAsp-hp

    Plasmid

    #48122

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    predicted optimal signal peptide for Bacillus megaterium protein secretion
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTFP1-H2A-10

    Plasmid

    #55488

    Purpose

    Localization: Nucleus/Histones, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    H2A (H2AC13 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPYngK+-hp

    Plasmid

    #48121

    Purpose

    Shuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    codon optimized signal peptide of YngK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. YEpURA-CUP1-HBTubiquitin

    Plasmid

    #26900

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

    Insert

    HBT-tagged ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. TIAM1_PDZ_1

    Plasmid

    #103925

    Purpose

    Protein expression and purification of TIAM PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. L_NES 67kDa

    Plasmid

    #201357

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    Adeno_NES-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. M_NLS 67kDa

    Plasmid

    #201351

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. H_NES 67kDa

    Plasmid

    #201350

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    HIV_NES-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. M_NES 67kDa

    Plasmid

    #201356

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    MAPK_NES-EGFP-6PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPpT4- HTA1prom-TIR1 F74G -FLAG

    Plasmid

    #189728

    Purpose

    Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    HTA1

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. NAX_CAGGS_CAS9_m4_VPR_Puro

    Plasmid

    #167887

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SLC9A3R1_PDZ_2

    Plasmid

    #103933

    Purpose

    Protein expression and purification of SLC9A3R1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

    Plasmid

    #149661

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC12), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PDLIM7_PDZ_1

    Plasmid

    #103940

    Purpose

    Protein expression and purification of ENIGMA PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM7 (PDLIM7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NAX_CAGGS_CAS9_m4_VPR_EYFP

    Plasmid

    #167882

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

    Plasmid

    #149588

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-C1X-Nup93-mut

    Plasmid

    #87335

    Purpose

    To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP93 (NUP93 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSPYngK-hp

    Plasmid

    #48118

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    signal peptide of YngK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge