We narrowed to 6,743 results for: GFP expression plasmids

1. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

   Plasmid

   #178047

   Purpose

   CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Lrig1 shRNAmir
   

   Use

   Cre/Lox and RNAi; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmGW182-12xmut_1-1115_P

   Plasmid

   #147159

   Purpose

   Insect Expression of DmGW182-12xmut_1-1115

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182-12xmut_1-1115 (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent and two non silent mutations V704A, N…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-EGFP-DmGW182_1-830-dsRNAres_N

   Plasmid

   #146972

   Purpose

   Insect Expression of DmGW182_1-830-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-830-dsRNAres (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-EGFP-DmGW182_1-1115-dsRNAres_N

   Plasmid

   #146973

   Purpose

   Insect Expression of DmGW182_1-1115-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182_1-1115-dsRNAres (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two non silent mutations V704A and N799S, and one…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-EGFP-DmGW182-delM2-dsRNAres_H

   Plasmid

   #146438

   Purpose

   Insect Expression of DmGW182-delM2-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmGW182-delM2-dsRNAres (gw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two non silent mutations V704A and N799S and one …

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW57.1-GFP_mmu-miR-292b-3p_sponge

   Plasmid

   #132622

   Purpose

   Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

   Plasmid

   #194247

   Purpose

   Expresses 3 miRNAs targeting human alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(human) (SNCA Human)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQdCas9.luxR(mut)-sggfp

   Plasmid

   #236185

   Purpose

   The plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   Quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Quorum sensing promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid

    #135241

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5-TurboID
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid

    #135264

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid

    #135263

    Purpose

    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-eGFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid

    #135240

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5-miniTurbo
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid

    #223504

    Purpose

    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    dead-mutant SNAP-tag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SNAP-tag C145A

    Promoter

    hSyn

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid

    #110378

    Purpose

    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    crystallin zeta (CRYZ Human)
    

    Use

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bik-L61G-pEGFP-C1

    Plasmid

    #177426

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Leucine 61 replaced with glycine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid

    #177428

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycine 154 replaced with STOP codon

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ST-GFP

    Plasmid

    #162500

    Purpose

    Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TR-GFP

    Plasmid

    #80591

    Purpose

    Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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