We narrowed to 1,414 results for: PUC57

1. pUCmini-iCAP-PHP.V1

   Plasmid

   #127847

   Purpose

   non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.V1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. LiOn-CAG∞IRFP

   Plasmid

   #154017

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   IRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TC1332

   Plasmid

   #164874

   Purpose

   CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCmini-iCAP-PHP.B

   Plasmid

   #103002

   Purpose

   non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

   Plasmid

   #205445

   Purpose

   human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   iCasp9-Crry-mCD59-Qa1 SCT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUCmini-iCAP-PHP.B3

   Plasmid

   #103004

   Purpose

   non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B3 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUCmini-iCAP-PHP.B2

   Plasmid

   #103003

   Purpose

   non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AA304

   Plasmid

   #216072

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid

    #154840

    Purpose

    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    HYGR∆ encodes aa 1-226

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid

    #71391

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. TC1681

    Plasmid

    #164872

    Purpose

    CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAVS1-PDL1

    Plasmid

    #159280

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    Human PDL1/CD274 (CD274 Human)
    

    Use

    AAV; S1 knockin donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TC1614

    Plasmid

    #164873

    Purpose

    CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A inserted into RfxCas13d (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid

    #205443

    Purpose

    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    hCD46-HLA-G SCT-hCD47-hCD59
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TC1316

    Plasmid

    #164883

    Purpose

    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    dcas13d-ADARdd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas13d L560-G586 deleted

    Promoter

    CMV, T7

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNKX2-5-T2A-GFP-PGK-Puro

    Plasmid

    #203607

    Purpose

    Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA031

    Plasmid

    #216011

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpRY (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA030

    Plasmid

    #216010

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpG (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA028

    Plasmid

    #216008

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9 (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge