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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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TC811

Plasmid

#164893

Purpose

TRE::YFP, inducible YFP expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

YFP

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-R2-pA-L4

Plasmid

#141062

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK-GW

Plasmid

#126576

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatible

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVKG1-BSD

Plasmid

#108680

Purpose

Blasticidin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

SV40pro-BSD-polyA

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4B

Plasmid

#102713

Purpose

Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-M-RAN-H2A2B

Plasmid

#106420

Purpose

M-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-H2A2B

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-H2A2B

Plasmid

#106421

Purpose

Y-RAN-H2A2B (RANbody H2A2B::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPMS008 (JBEI-11596)

Plasmid

#87957

Purpose

jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, ColE1origin

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pKS013

Plasmid

#65466

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
CMJ162

Plasmid

#69527

Purpose

pTET etuAld+Ile1-20-pduD21-224- FLAG

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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CMJ159

Plasmid

#69525

Purpose

pTET HochAld1-20-pduD21-224- FLAG

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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CMJ157

Plasmid

#69523

Purpose

pTET eutE1-20-pduD21-224-FLAG

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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CMJ156

Plasmid

#69522

Purpose

pTET eutC1-20-pduD21-224-FLAG

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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