We narrowed to 3,318 results for: psin

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AAV1-DIO-eGFP-pvRPL10a

Plasmid

#202542

Purpose

Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.

Depositor

Zoe Donaldson

Article

Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75

Insert

RPL10a subunit of the ribosome

Use

AAV and Cre/Lox

Tags

eGFP

Expression

Mammalian

Mutation

optimized for prairie vole DNA sequence and optim…

Promoter

human synapsin

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-fwd[Chrimson-tdT]

Plasmid

#59137

Purpose

Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-tdTomato

Use

Cre/Lox

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55648

Purpose

Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR

Plasmid

#137950

Purpose

Expresses iAChSnFR under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1

Insert

iAChSnFR

Use

AAV

Expression

Mammalian

Promoter

hSynanpsin

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-rtTAV16

Plasmid

#102367

Purpose

AAV vector to drive TET-ON activator expression from human synapsin I promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Front Neural Circuits. 2014 Sep 17;8:110

Insert

rtTAV16

Use

AAV

Expression

Mammalian

Mutation

rtTA variant reported in Gene Therapy (2006) 13, …

Promoter

human synapsin I promoter

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-mCerulean

Plasmid

#154951

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Has Service

AAV Retrograde and AAV9

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

Plasmid

#35503

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

Plasmid

#106180

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV5

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chrimson-GFP

Plasmid

#62718

Purpose

AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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hSyn-GtACR2-ChRmine-tandem-eYFP

Plasmid

#192580

Purpose

AAV-vector for bidirectional optogenetic manipulation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

somBiPOLES

Use

AAV

Tags

EYFP- soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

Plasmid

#177345

Purpose

AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

dead hSaCas9

Use

AAV and CRISPR

Tags

3x GCN4 peptide, 3xHA, and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

Synapsin

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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ITIH4-bio-His

Plasmid

#52181

Purpose

Expresses full-length Inter-alpha-trypsin inhibitor heavy chain H4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITIH4 (ITIH4 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mOrange-P2A-hKvb1

Plasmid

#154084

Purpose

Independent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoter

Depositor

Michael Hoppa

Article

Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

Insert

mOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)

Use

AAV

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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