We narrowed to 1,755 results for: quo;
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Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-m
Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorInsertABE8.17-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-m
Plasmid#136296Purposeexpresses ABE8.13-m in mammalian cellsDepositorInsertABE8.13-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CAG-GFP
Plasmid#83279Purposerecombinant AAV vector packaging self-complementary GFP under the CAG promoterDepositorInsertenhanced green fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHluorin
Plasmid#70113PurposeExpression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidificationDepositorInsertlyso-pHluorin (Cd63 Aequorea victoria, Mouse)
UseTagsHA-Tag and pHluorinExpressionMammalianMutationPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-tetOn-IRES-G418 (EtO)
Plasmid#84776PurposeSecond generation lenti tetracycline operator (on)DepositorInsertTET OPERATOR
UseLentiviralTagsExpressionMutationPromoterpTIGHTAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…ExpressionMutationPromoterSynapsinAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2
Plasmid#69624PurposeA FRET sensor for Galphai2 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTq2-Calmodulin
Plasmid#198200PurposeFRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2DepositorInsertCalmodulin 2 (Calm2 Rat)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2
Plasmid#69623PurposeA FRET sensor for Galphai1 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pGFAP-AcGFP
Plasmid#133733PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpGFAP and pTRE3G-BiAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCMV and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
UseTagsExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Human, Aequorea victoria, Fly)
UseTagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …PromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
1179_pAAV-U6-BbsI-gRNA-CB-EmGFP
Plasmid#89060PurposeAAV-gRNA cloning vector with GFP reporterDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only