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Anti-TrpV1 [N221/17R]

Plasmid

#177517

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.4 K+ channel [K13/31R]

Plasmid

#128613

Purpose

Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (Rat). Derived from hybridoma K13/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.4 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcna4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.EZH2

Plasmid

#125171

Purpose

Expresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Tags

His-TEV

Expression

Insect

Promoter

Polyhedrin

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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MEK1-pcw107-V5

Plasmid

#64650

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Anti-QKI-7 [N183/15R]

Plasmid

#177507

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human). Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

Plasmid

#105801

Purpose

Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: FLAG-3C; ORF2: mClover3-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ERalpha (Y537S mutant)-pcw107-V5

Plasmid

#64634

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

ESR1 (transcript variant 1) (ESR1 Human)

Use

Lentiviral

Tags

V5

Mutation

Y537S

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Stat3 (A662C,N664C,V667L)-pcw107-V5

Plasmid

#64611

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Tags

V5

Mutation

A662C, N664C, V667L

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) FLAG-Ago2 S387E

Plasmid

#60653

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

CMV-FLAG-Ago2 (N-term tag)

Expression

Mammalian

Mutation

S387E mutant

Promoter

CMV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-Npas4 [N408/79]

Plasmid

#177547

Purpose

Mammalian Expression Plasmid of anti-Npas4 (Rat). Derived from hybridoma N408/79.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Npas4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Npas4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Gli2 truncation-pcw107-V5

Plasmid

#64626

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

GLI2 (truncation mutant) (GLI2 Human)

Use

Lentiviral

Tags

V5

Mutation

truncated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
Anti-nNOS/NOS1 [L121/133R]

Plasmid

#188170

Purpose

Mammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/133

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Rgl2-CAAX-pcw107-V5

Plasmid

#64641

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Rgl2 plus C-term KRAS (Kras Mouse)

Use

Lentiviral

Tags

V5

Mutation

C-term of Kras

Promoter

PGK

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only
Anti-EAAC1 [N420/24R]

Plasmid

#206572

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N420/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc1a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-EAAC1 [N180/41R]

Plasmid

#206615

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N180/41.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc1a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GIT2 [N83/48R]

Plasmid

#140079

Purpose

Mammalian Expression Plasmid of anti-GIT2 (Human). Derived from hybridoma N83/48.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GIT2 (Human) recombinant mouse monoclonal antibody (GIT2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/42]

Plasmid

#190488

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/42.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) FLAG-Ago2 S387A

Plasmid

#60652

Purpose

Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

CMV-FLAG-Ago2 (N-term tag)

Mutation

S387A mutant

Promoter

CMV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV1 [N221/12R]

Plasmid

#188224

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/12

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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