We narrowed to 61,789 results for: %s
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Plasmid#176112PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
ExpressionMammalianAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-Sir2a
Plasmid#122510PurposeCombining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.DepositorInsertdCas9-Sir2a
UseCRISPRTags3*FLAGMutationdCas9(D10A,H840A)PromoterPfHsp86Available SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107318PurposeExpresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-DR-cr in PBCSK-
Plasmid#126640PurposeCloning vector AsCas12a DR-crRNA for expression in mammalian cellsDepositorInsertAsCas12a Full Length Direct Repeat crRNA
UseCRISPRExpressionMammalianPromoterU6 PromoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase full length (2135)
Plasmid#86656PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation2120 base pairs including up to coding nucleotide…PromoterCDK4Available SinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gDNMT1.Cas9-T2A-GFP
Plasmid#170362PurposePlasmid used for Crispr/cas9 based disruption of human DNMT1.DepositorInsertCas9-T2A-GFP
UseLentiviralAvailable SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENExpressionMammalianPromoterCAGGSAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENExpressionMammalianPromoterCAGGSAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
Tags14xHis on Csf3ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
Tags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a
Plasmid#79592PurposeTransient and retroviral expressionDepositorAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-myc3-FBW5 delta F
Plasmid#19912DepositorInsertF-box and WD repeat domain containing 5 delta F (FBXW5 Human)
Tagsmyc3ExpressionMammalianMutationdeleted aa1-42 (F-box)Available SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
cpGFP-Adhiron_flex
Plasmid#165044PurposeFlexible linker control for POLArISact expression in mammalian cellsDepositorInsertcpGFP-Adhiron (connected with a flexible linker)
TagsHAExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)
Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1ExpressionMammalianAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
TagsEYFP, His-tagExpressionBacterialPromoterT7Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SIMx6
Plasmid#116938PurposeBacterial expression vector for 6 simDepositorInsert6 sim
ExpressionBacterialPromoterT7Available SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-HISx6-SUMOx10
Plasmid#116937PurposeBacterial expression vector for 10 sumoDepositorInsert10 SUMO
TagsHISExpressionBacterialPromoterT7Available SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only