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EGFP-uTEV1-MAVS-mTA3

Plasmid

#171001

Purpose

HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIV]

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_L6_mKate2

Plasmid

#167332

Purpose

Expresses mKate2 fused to TFAM L6 mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K136, H137, K139, R140, K146 and K147 to …

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-C4A,C5A,C7A-GFP

Plasmid

#166838

Purpose

Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

C4A, C5A, C7A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2F823D

Plasmid

#165105

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d F823D

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

Insert

Nedd4.2d F823D (NEDD4L Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression; F823D; …

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA sCRNA 2.0 (GB2461)

Plasmid

#160593

Purpose

Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA sCRNA 2.0

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDUAL_2_lacZ

Plasmid

#155173

Purpose

see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_araBAD

Plasmid

#155172

Purpose

BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_2_tacI

Plasmid

#155174

Purpose

see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL_lacZ

Plasmid

#155169

Purpose

BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBICI_2_medium

Plasmid

#155167

Purpose

see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBICI_strong

Plasmid

#155165

Purpose

BaSP and CuCBP co-expression: strong RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBICI_medium

Plasmid

#155164

Purpose

BaSP and CuCBP co-expression: medium RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBICI_weak

Plasmid

#155163

Purpose

BaSP and CuCBP co-expression: weak RBS controls BaSP expression

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7(zebrafish) C49S

Plasmid

#108914

Purpose

Express C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 49 to Serine

Promoter

SP6

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Oct4deltaPE-53BP1mCherry

Plasmid

#141122

Purpose

Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

Depositor

John Schimenti

Article

Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

Insert

Fragment of human 53BP1 (TP53BP1 Human)

Use

Mouse Targeting

Tags

mCherry

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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TB:3xDBE_RE:MiniP

Plasmid

#124529

Purpose

3 Copies of the Daf-16 family binding element

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

3 copies of the Daf-16 family binding element

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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phNFL-L268P

Plasmid

#132593

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E L268P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

803T>C; Leu268Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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phNFL-P8R

Plasmid

#132590

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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phNFL-P22S

Plasmid

#132591

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

64C>A, 65C>G; Pro22Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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