We narrowed to 59,466 results for: %s

1. pEXPqcxip-hFTSJ3-FLAG

   Plasmid

   #165463

   Purpose

   Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
   

   Use

   Retroviral; Gateway

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV/MSV, 5'LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

   Plasmid

   #178528

   Purpose

   Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   rtTA, SspB-mScarlet-I-MYPT (1-169aa)
   

   Expression

   Mammalian

   Promoter

   CAG, Tet responsive element

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNICKclos2.0

   Plasmid

   #73228

   Purpose

   Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sgRNA to xylR
   

   Use

   E.coli - clostridium shuttle vector

   Mutation

   D10A

   Promoter

   Pj23119 and Pthl

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-HE-Geminin

   Plasmid

   #109402

   Purpose

   Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

   Depositor

   Jean-Paul Concordet

   Article

   Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

   Insert

   HE-Geminin
   

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-GBP-MTn

   Plasmid

   #154363

   Purpose

   Bacterial expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-PIPK1 gamma 87

   Plasmid

   #22300

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-M4-AR-S81A

   Plasmid

   #171241

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

   Depositor

   Steven Balk

   Article

   Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

   Insert

   3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser81Ala (AR-S81A)

   Promoter

   CMV

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1delZn2

   Plasmid

   #173935

   Purpose

   Bacterial expression of PARP1 lacking Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 (V762A)

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SlugCas9-HF

    Plasmid

    #163796

    Purpose

    Expresses highly specific SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    SlugCas9 (R247A, N415A, T421A, R656A)

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-TetO-Junb

    Plasmid

    #178441

    Purpose

    doxycycline-inducible expression of Mouse Junb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Junb (Junb Mouse)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1P CISD1_2

    Plasmid

    #160774

    Purpose

    Suppress CISD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shCISD1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX SH3(2)-3R

    Plasmid

    #112091

    Purpose

    Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-3R (NCK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1-ART

    Plasmid

    #174810

    Purpose

    Bacterial expression of isolated PARP1 ART subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-ART (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-BRCT

    Plasmid

    #174807

    Purpose

    Bacterial expression of isolated PARP1 BRCT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-BRCT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-WGR

    Plasmid

    #174808

    Purpose

    Bacterial expression of isolated PARP1 WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-WGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-puro-tetON-shRFP

    Plasmid

    #110940

    Purpose

    Tet-inducible shRNA targeting RFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shRFP
    

    Use

    Lentiviral and RNAi

    Promoter

    H1/TO

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV106-pTDH3-mCherry-10xSumo-6xSIM

    Plasmid

    #116928

    Purpose

    Yeast integrative vector for expression of the SUMO-SIM complex

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10xSUMO-6xSIM
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-mDyn2 pcDNA3

    Plasmid

    #36264

    Depositor

    Pietro De Camilli

    Article

    Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

    Insert

    Dynamin 2 (Dnm2 Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid

    #189630

    Purpose

    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV, Cre/Lox, and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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