We narrowed to 57,432 results for: %s

1. attB-­mCh-­Poly(A)‐NeoR

   Plasmid

   #65528

   Purpose

   NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-mCherry
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai35

   Plasmid

   #34882

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   CAG-Floxed Archaerhodopsin3-GFP
   

   Use

   Mouse Targeting

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDECKO_mCherry_Rat_cebp-alfa

   Plasmid

   #140098

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry_SPI1

   Plasmid

   #140099

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)

   Plasmid

   #74505

   Purpose

   Overexpression of CSK SH3-SH2 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   SH3-SH2 domains

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1(-)-V5.hOr47a

   Plasmid

   #126474

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-)-V5.mER.hOr47a

   Plasmid

   #126473

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-Neo-PTPRT (R632X)

   Plasmid

   #16629

   Depositor

   Bert Vogelstein

   Article

   Wang et al Science. 2004 May 21. 304(5674):1164-6.

   Insert

   PTPRT R632X (PTPRT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncated R632X mutant

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pLD-puro-PGKnVA

   Plasmid

   #24589

   Depositor

   Jason Moffat

   Article

   Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

   Insert

   Gateway(TM) cassette
   

   Use

   Lentiviral

   Tags

   VA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pYPQ256

    Plasmid

    #124312

    Purpose

    Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Insert

    pcoCas9D10A-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP1-N1-RvCAHS8-GS

    Plasmid

    #192461

    Purpose

    Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shCTL-mlpx-puro

    Plasmid

    #65232

    Purpose

    encodes a non-targeting control shRNA

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    non-coding shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA pDEST40 Cas9 GW STOP

    Plasmid

    #183196

    Purpose

    Destination vector

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmirGLO-Bmpr2-Δ17

    Plasmid

    #49380

    Purpose

    Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Bmpr2 (BMPR2 Human, Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    changed nucleotides AC to TG at bp 7347-7348

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-A2-77p-Venus

    Plasmid

    #64856

    Purpose

    Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

    Depositor

    Sriram Neelamegham

    Article

    Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

    Insert

    VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
    

    Use

    Tags

    7X His and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV V5-nurim

    Plasmid

    #175110

    Purpose

    Lentiviral expression of V5-tagged mouse Nrm

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Nrm (Nrm Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

    Plasmid

    #112211

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    C-terminus of dCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRShygro shID3#1

    Plasmid

    #19164

    Depositor

    Joan Massague

    Article

    Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

    Insert

    small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2008

    Availability

    Academic Institutions and Nonprofits only