165,271 results

1. pENN239_CRISPRcharm_Kv1

   Plasmid

   #220838

   Purpose

   CRISPRcharm Kv1 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv1
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1+U1snRNA

   Plasmid

   #194202

   Purpose

   Expresses U1 snRNA

   Depositor

   Matthias Leisegang, Ralf Brandes

   Insert

   U1 snRNA (RNU1-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP STAT1 WT

   Plasmid

   #12301

   Depositor

   Alan Perantoni

   Article

   Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

   Insert

   STAT1 (STAT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6TEV-UbG76C

   Plasmid

   #75299

   Purpose

   Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   human Ubiquitin mutant G76 to C
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Glycine 76 to Cystine

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

   Plasmid

   #79671

   Purpose

   Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dendra2-CathespinB-6

   Plasmid

   #57703

   Purpose

   Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Insert

   CathespinB
   

   Tags

   Dendra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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