159,530 results

1. pUC19-hSNRPN-R-loop

   Plasmid

   #134902

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   hSNRPN promoter (SNRPN Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p413 TEF NAPstarC

   Plasmid

   #232737

   Purpose

   Expresses control sensor NAPstarC in S. cerevisiae.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstarC
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92390

   Purpose

   expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHMR1A

   Plasmid

   #102957

   Purpose

   gene cloning of MR1A

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

   Insert

   MR1A cDNA (MR1 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Stop codon has been removed by HindIII site

   Promoter

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

   Plasmid

   #125696

   Purpose

   Global labeling of inhibitory synapses (red fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   mRuby2-Gephyrin.FingR-IL2RGTC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKI074

   Plasmid

   #11571

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGST2-GST-TEV-OPTN

   Plasmid

   #198428

   Purpose

   Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   GST and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-BAF

    Plasmid

    #227442

    Purpose

    Mammalian expression of GFP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ERG_pet28a

    Plasmid

    #131642

    Purpose

    Bacterial expression of ETS gene ERG

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ERG (ERG Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. UKNP2.4.1

    Plasmid

    #98212

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSICO-eGFP-TNPO3

    Plasmid

    #167590

    Purpose

    Expresses human TNPO3 fused to eGFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    eGFP-TNPO3 (TNPO3 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    WT

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEos3.2-H1-10

    Plasmid

    #57457

    Purpose

    Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    H1 (H1f0 Mouse)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDrVSP(C302S)-IRES2-EGFP

    Plasmid

    #80331

    Purpose

    Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

    Depositor

    Yasushi Okamura

    Article

    Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

    Insert

    Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C302S

    Promoter

    CMV

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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