We narrowed to 12,204 results for: 112

1. CSK gRNA (BRDN0001149085)

   Plasmid

   #77767

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CSK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CSK (CSK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2-WT(732)

   Plasmid

   #154098

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

   Plasmid

   #193717

   Purpose

   Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
   

   Tags

   2xmNeongreen-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF164

   Plasmid

   #135928

   Purpose

   All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Insert

   TNFRSF9 fused to mOrange
   

   Use

   Transposon

   Tags

   mOrange

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF160

   Plasmid

   #135925

   Purpose

   All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Insert

   TNFRSF9 fused to mAzurite
   

   Use

   Transposon

   Tags

   mAzurite

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Flt3-Y591F/Y919F

   Plasmid

   #74498

   Purpose

   Retroviral expression of FLT3 Y591F/Y919F

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Y591F/Y919F, T227M

   Promoter

   unknown

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Venus-Bik-4E-pEGFP-C1

   Plasmid

   #166744

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bik-4E (BIK Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EFS-DIO-GRAB_CRF1.0

   Plasmid

   #208662

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

   Plasmid

   #145842

   Purpose

   Lentiviral vector genome to ectopically express human Ace2

   Depositor

   Caroline Goujon

   Article

   Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

   Insert

   human Ace2 (ACE2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-plk1-AKAP

    Plasmid

    #132967

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
    

    Expression

    Mammalian

    Mutation

    C terminus of full-length Plk1 fused to the peric…

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOmnibac_ZZ_linker_ybbR_MAP7

    Plasmid

    #159718

    Purpose

    Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    MAP7 (Microtubule Associated Protein 7) (MAP7 Human)
    

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid

    #193720

    Purpose

    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-GG-hUbC-EBPF2-gRNA-TGFB2

    Plasmid

    #185556

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    TGFB2 gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6-N-3XFLAG-Gp78

    Plasmid

    #123590

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307-Bat-CoV-HKU9-3CL-C144A

    Plasmid

    #160285

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CSK gRNA (BRDN0001144968)

    Plasmid

    #77766

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSK (CSK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTV001

    Plasmid

    #130265

    Purpose

    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA::His8
    

    Tags

    polyhistidine (internal)

    Expression

    Bacterial

    Mutation

    8His polyhistidine encoding region and XhoI site …

    Promoter

    araBAD

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-GG-hUbC-EBPF2-gRNA-MFAP2

    Plasmid

    #185555

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    MFAP2 gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag‐mTim3 (del.Cyto)

    Plasmid

    #49209

    Purpose

    expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (del.Cyto) (Havcr2 Mouse)
    

    Tags

    CD8 signal sequence and FLAG

    Expression

    Mammalian

    Mutation

    cytosolic domain deleted, no Tim3 signal sequence…

    Promoter

    Ef1a

    Available Since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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