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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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D9_Ld_190920

Plasmid

#135825

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2

Plasmid

#124063

Purpose

Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXIATL2

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGal3

Plasmid

#73080

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin-3 (LGALS3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3

Plasmid

#175778

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

Plasmid

#156447

Purpose

For transient expression of mouse SMC3 tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SMC3 (Smc3 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

Plasmid

#182651

Purpose

human CD81-TEV cleavage site-FKBP-SmBiT tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)

Tags

SmBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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FUG-Pten

Plasmid

#140732

Purpose

Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-Pten (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

hUbiC

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN-LONG

Plasmid

#49417

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN-Long (PTEN )

Expression

Bacterial

Promoter

T5

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP11

Plasmid

#65830

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP11

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pWZ699

Plasmid

#208117

Purpose

Payload yeast assembly vector with TK-mActb on backbone

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Type

Empty backbone

Use

Synthetic Biology; Copy number inducible

Expression

Bacterial, Mammalian, and Y…

Promoter

Human PGK1 for thymidine kinase

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRM055 His-TEV-Cas9 6xNLS 0C

Plasmid

#196246

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS3

Plasmid

#175756

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode

Plasmid

#170248

Purpose

Barcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

C124S

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-GATA2

Plasmid

#131602

Purpose

expression of human GATA2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

GATA2 (GATA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-mSema4D

Plasmid

#190645

Purpose

Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4d (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Expression

Mammalian

Mutation

R186S

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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