We narrowed to 64,006 results for: cell

1. pGFP-Ub-VPS13D_dC

   Plasmid

   #176466

   Purpose

   express VPS13D lacking C-terminal 407 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   C-terminal 407 aa is deleted.

   Promoter

   CMV

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LB1361 (Bcl-2 promoter with MHS12)

   Plasmid

   #15391

   Depositor

   Linda Boxer

   Article

   Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Bcl-2-Luciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Bcl-2 full promoter with MHS12

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Xl-VSP1 R152Q GFP

   Plasmid

   #51884

   Purpose

   expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

   Plasmid

   #173214

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-tetO-Nfat1

   Plasmid

   #139830

   Purpose

   doxycycline-inducible expression of mouse Nfat1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Nfat1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PR594-His-bio

   Plasmid

   #52332

   Purpose

   Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PR594 (NSG1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN65

   Plasmid

   #100123

   Purpose

   expresses putative SOX2 DBD with a V5 tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc-UVRAG S498D

   Plasmid

   #86739

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human UVRAG (UVRAG Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S498D

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP-GTSE1(638-720)-GFP

   Plasmid

   #100735

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   GTSE1 (GTSE1 Human)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   C-terminus only

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBI-CMV1-mir451-CMV2-mir1~144

    Plasmid

    #164665

    Purpose

    Express miR-451 and miR-1-1~144 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-451a (MIR451A Human)
    miR-1-1~144 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN16

    Plasmid

    #100103

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-PCNA-19-NLS-4

    Plasmid

    #54222

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Tags

    SV40NLS and mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pDEST-CMV GFP-GABARAPL1 G116A

    Plasmid

    #123118

    Purpose

    Expresses EGFP-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF Itk K390R myc

    Plasmid

    #20707

    Depositor

    Leslie Berg

    Article

    Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

    Insert

    Itk K390R (Itk Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Kinase Dead. K390R.

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pClneoFH-let60 G12V

    Plasmid

    #66860

    Purpose

    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    Let-60 (let-60 Nematode)
    

    Tags

    FLAG-His6

    Expression

    Mammalian

    Mutation

    Glycine 12 is mutated to valine

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pUCHR-mClover-smAID-P2A-CD5HA2-bglpA

    Plasmid

    #110655

    Purpose

    Lentiviral vector expressing cell surface HA-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5HA2-bglpA
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN20

    Plasmid

    #100105

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'medium' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3604 pGEX4T-1 Bad S136A (104-141)

    Plasmid

    #8801

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Mol Cell 1999 Apr;3(4):413-22.

    Insert

    Bad (Bad Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains AA's 104-141; S136A

    Promoter

    Ptac

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. SGCD-His-bio

    Plasmid

    #52333

    Purpose

    Expresses full-length Delta-sarcoglycan ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SGCD (SGCD Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMiNGo11-3

    Plasmid

    #126058

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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