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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #1

Plasmid

#27016

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #1 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pECFP-histone H3

Plasmid

#179698

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Histone H3 (H3c10 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#233539

Purpose

A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#233541

Purpose

A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT38

Plasmid

#223410

Purpose

T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Sox2

Plasmid

#19765

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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EK0304 CMV-TO-BFPmut-stop-Bdnf.t1 (FLP-IN)

Plasmid

#191146

Purpose

Expression of only the trigger sequence for Bdnf.s1 (EK0301) in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP3-Halo

Plasmid

#175310

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP3 (IGF2BP3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE3-VP64-P10

Plasmid

#104607

Purpose

Expresses TALE3 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE3

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSEM271 - MCS mosTI NeoR target

Plasmid

#159830

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI NeoR target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN REP5-GFP-GluR2 (Q)

Plasmid

#24003

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR2 (Gria2 Rat)

Use

Sindbis virus production

Tags

GFP

Mutation

R607Q

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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phCMV-IE1

Plasmid

#118048

Purpose

GBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Cytomegalovirus enhancer and promoter

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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