We narrowed to 13,228 results for: 109

1. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF

   Plasmid

   #236153

   Purpose

   Express mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   SCN1A-377-deImmLink-NZF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G2E, A104K, S109I in SCN1A-377

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

   Plasmid

   #39303

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q109A, W110A, K112A and K113A

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184740

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSS

   Plasmid

   #39272

   Depositor

   Peter Kast

   Article

   Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

   Type

   Empty backbone

   Use

   Positive-selection cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Recall-miniCMV-sfGFP

   Plasmid

   #137995

   Purpose

   Backbone for generation of ClonMapper Recall Plasmid

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 5XDRE-luciferase

   Plasmid

   #85623

   Purpose

   Luciferase reporter containing five tandem copies of the core Dpp response element

   Depositor

   Kenneth Irvine

   Article

   Oh et al Dev Cell. 2011 Jan 18;20(1):109-22. doi:

   Insert

   5x DRE
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA.PKD

   Plasmid

   #10808

   Depositor

   Alex Toker

   Article

   Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

   Insert

   PKD (PRKD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L520P

   Promoter

   CMV

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA.PKD.Y463F

    Plasmid

    #10813

    Depositor

    Alex Toker

    Article

    Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

    Insert

    PKD (PRKD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y463F, inactive

    Promoter

    CMV

    Available since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pLVPT-rtTR-KRAB

    Plasmid

    #11777

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB, Tet-off
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSUPER.PKD1.RNAi

    Plasmid

    #10815

    Depositor

    Alex Toker

    Article

    Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

    Insert

    PKD1 RNAi (PRKD1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVU-tTR-KRAB

    Plasmid

    #11645

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, no tetO
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ExRai-PKNAR

    Plasmid

    #226631

    Purpose

    Excitation-ratiometric biosensor for monitoring Protein Kinase Novel activity in living cells

    Depositor

    Ju Chen

    Article

    Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

    Insert

    ExRai-PKNAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLJM60-Tia1

    Plasmid

    #38243

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Tia1 (Tia1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

    Plasmid

    #137993

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PM-ExRai-PKNAR

    Plasmid

    #226633

    Purpose

    Plasma membrane-targeted ExRai-PKNAR

    Depositor

    Ju Chen

    Article

    Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

    Insert

    PM-ExRai-PKNAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasma membrane targeting N-terminal 11 amino aci…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PM-ExRai-PKNAR(T/A)

    Plasmid

    #226634

    Purpose

    Negative-control mutant for PM-ExRai-PKNAR biosensor

    Depositor

    Ju Chen

    Article

    Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

    Insert

    PM-ExRai-PKNAR(T/A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Phosphorylatable Thr of PM-ExRai-PKNAR mutated to…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge