Addgene

We narrowed to 8,183 results for: FIE

Showing: 1121 - 1140 of 8183 results

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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-BioID2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-hXRCC1

Plasmid

#52274

Purpose

Bacterial expression of human XRCC1 with N-terminal GST-tag

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMCB306

Plasmid

#89360

Purpose

sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEntr-GATA4flbio

Plasmid

#32937

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

GATA4 (Gata4 Mouse)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

none

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_human

Plasmid

#71509

Purpose

humanSTARR-seq screening vector with SCP1 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Humanstarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pAV0679

Plasmid

#149689

Purpose

Bacterial expression of SENP_EuH protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuH protease

Use

Tags

His14-tag and Tev protease cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMP71-CD40L

Plasmid

#174595

Purpose

Stably expresses the extracellular and transmembrane domain of murine CD40L

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD40L

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p155 pCMV-EGFP/beta-gal

Plasmid

#8387

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCre-ERT2

Plasmid

#128168

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PAPGSTMA N-terminus)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Use

Tags

His6 and V5

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly

Plasmid

#71499

Purpose

flySTARR-seq screening vector with DSCP core promoter.

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Mutation

Promoter

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-NS

Plasmid

#109729

Purpose

Multisite gateway vector for 5' tagging with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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