We narrowed to 13,944 results for: cas9

1. pBFv-nosP-Cas9

   Plasmid

   #138402

   Purpose

   Expresses Cas9 in the germline of Drosophila

   Depositor

   Shu Kondo

   Article

   Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

   Insert

   Cas9-NLS
   

   Expression

   Insect

   Promoter

   UAS

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459V2.0-HypaCas9-mRuby2

   Plasmid

   #183872

   Purpose

   pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HypaCas9-P2A-mRuby2-T2A-Puro

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas9-mD3A

   Plasmid

   #78257

   Purpose

   Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   E756A

   Promoter

   pCMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1289-AAV-dCjCas9

   Plasmid

   #223138

   Purpose

   AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-dCas9-TET1-Hygro

   Plasmid

   #104404

   Purpose

   transient expression of dCas9-TET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   TET1 (TET1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLO 9.3_bCas9-CP230

   Plasmid

   #121497

   Purpose

   TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   p15A

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3s-Sniper-Cas9

    Plasmid

    #113912

    Purpose

    Expresses Sniper-Cas9 with CMV promoter

    Depositor

    Jungjoon Lee

    Article

    Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

    Insert

    Sniper-Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid

    #165424

    Purpose

    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    

    Use

    Synthetic Biology

    Promoter

    Os Ubiquitin promoter :: Cas9 :: NosTerminator

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. modRNAc1-Cas9-2A-puro

    Plasmid

    #216390

    Purpose

    this vector can be used to make modRNA expressing Cas9 and puro resistance gene

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    Cas9-2A-puro
    

    Use

    CRISPR

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-PuroR_hNT

    Plasmid

    #71830

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    Non-targeting sgRNA human (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPIK16045_pX330_Flag-SuperFi-Cas9

    Plasmid

    #184370

    Purpose

    Mammalian expression of SuperFi-Cas9 nuclease

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

    Promoter

    CBh

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113343

    Purpose

    Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    p300core-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. IVT-nCas9(H840A)

    Plasmid

    #207455

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    H840A
    

    Expression

    Bacterial

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTK Cas9-2A-Citrine

    Plasmid

    #92393

    Purpose

    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    thymidine kinase promoter

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid

    #199255

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1) nuclease

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

    Insert

    High specificity eSpCas9(1.1) nuclease
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    K848A K1003A R1060A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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