We narrowed to 59,329 results for: cloning

1. pHBJT002

   Plasmid

   #225153

   Purpose

   High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r

   Plasmid

   #162936

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Triple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r

   Plasmid

   #162937

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C514-E11: attB5r-RU5u-His6-nostop-attB1r

   Plasmid

   #162953

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

   Plasmid

   #162956

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Halotag fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r

   Plasmid

   #162935

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Maltose-binding protein fusion, AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C514-E09: attB5r-RU5u-FLAG-nostop-attB1r

   Plasmid

   #162951

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   FLAG epitope tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

   Plasmid

   #162966

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Dual HA epitope tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C514-E42: attB5r-RU5u-Strep2-nostop-attB1r

   Plasmid

   #162961

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Strep2 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C514-E26: attB5r-RU5u-myc-nostop-attB1r

    Plasmid

    #162960

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Myc epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C514-E10: attB5r-RU5u-HA-nostop-attB1r

    Plasmid

    #162952

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C514-E24: attB5r-RU5u-V5-nostop-attB1r

    Plasmid

    #162959

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    V5 epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

    Plasmid

    #162934

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

    Plasmid

    #162933

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

    Plasmid

    #162932

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6 fusion with AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R777-E054 Hs.EGFR-nostop

    Plasmid

    #70338

    Purpose

    Gateway ORF clone of human EGFR [NM_005228.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R777-E075 Hs.EXOC5

    Plasmid

    #70359

    Purpose

    Gateway ORF clone of human EXOC5 [NM_006544.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    EXOC5 (EXOC5 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R777-E011 Hs.ARHGAP35

    Plasmid

    #70295

    Purpose

    Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    ARHGAP35 (ARHGAP35 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R777-E111 Hs.IRS2

    Plasmid

    #70395

    Purpose

    Gateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    IRS2 (IRS2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R777-E112 Hs.IRS2-nostop

    Plasmid

    #70396

    Purpose

    Gateway ORF clone of codon optimized human IRS2 [NM_003749] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    IRS2 (IRS2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge