We narrowed to 2,342 results for: clu

1. pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald

   Plasmid

   #225942

   Purpose

   Lentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Evan Reid, Richard Eva

   Inserts

   RRBP1 (RRBP1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

   Plasmid

   #120562

   Purpose

   Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   KRAS4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   CMV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-TetOminiCMV-OS

   Plasmid

   #136576

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

   Plasmid

   #120560

   Purpose

   Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

   Plasmid

   #140203

   Purpose

   Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   crRNA array elcA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

   Plasmid

   #169166

   Purpose

   Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

   Insert

   3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-FOXC1-T2A-miRFP670

   Plasmid

   #182336

   Purpose

   Expresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Forkhead Box C1 (FOXC1 Human)
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

   Plasmid

   #225584

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-F8.2 (S54L+T127A)-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T21R-F8.2 (S54L+T127A)

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid

    #225949

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C100A

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid

    #225950

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Additional cysteine inserted after Cys-100

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid

    #225955

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Truncated CKAP4 (1-131) lacking the luminal domain

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid

    #225938

    Purpose

    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    RTN4 (RTN4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

    Plasmid

    #225940

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Evan Reid, Richard Eva

    Inserts

    STIM2 (STIM2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDV18 [punc-47::SNG-1::CRY2olig(535)]

    Plasmid

    #197598

    Purpose

    Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    E490G

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDV12 [psng-1::SNG-1::CRY2olig(535)]

    Plasmid

    #197596

    Purpose

    Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    E490G

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid

    #215026

    Purpose

    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4X E-box mutations within Coordinator motifs

    Promoter

    SV40

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDV06 [punc-17::SNG-1::CRY2(535)]

    Plasmid

    #197597

    Purpose

    Expression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

    Insert

    Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    E490G

    Promoter

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-SOX2-T2A-miRFP670

    Plasmid

    #187375

    Purpose

    Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Tags

    T2A-miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

    Plasmid

    #146039

    Purpose

    Insect Expression of DmTral-FDF-ADA

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-FDF-ADA (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    FDF to ADA (407-409) mutation. Additionally, thre…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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