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lentiSAMv2 CAMK1D TSS-guide2

Plasmid

#118193

Purpose

CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA_NEPTUNE_hTLN2-FL

Plasmid

#164842

Purpose

Expression of NEPTUNE tagged FL human talin2

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-NEPTUNE

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-mito

Plasmid

#168304

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-mito

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAJF1137

Plasmid

#252907

Purpose

Tet-inducible TurboID-NBALFA fusion plasmid. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

TurboID

Expression

Bacterial

Promoter

Tet-inducible promoter

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-4V5-APEX2-URA

Plasmid

#207063

Purpose

Backbone plasmid containing ADH3 promoter and 4V5-APEX2 tag.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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HyPer7.2DAAO-NES

Plasmid

#168301

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NES

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ptet-RFP-shR-rtTA

Plasmid

#35625

Purpose

Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

AAV and RNAi; Tet inducible

Expression

Mammalian

Promoter

Ptet

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS (L4-R1)

Plasmid

#61374

Purpose

5' Gateway Entry Vector Containing QUAS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QUAS

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pQC-CherryOFF-GFP

Plasmid

#129101

Purpose

Reporter vector

Depositor

Fangliang Zhang

Article

Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

Insert

CherryOFF

Use

Retroviral

Expression

Mammalian

Mutation

G-> A

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_hTLN2-FL

Plasmid

#164840

Purpose

Expression of GFP tagged FL human talin2

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSXL101

Plasmid

#247078

Purpose

pZE12-luc containing MNX1 from Candida parapsilosis CBS604 and ubiC from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

MNX1
ubiC
AS

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Tgl3-4V5-APEX2-URA

Plasmid

#207066

Purpose

Expressing Tgl3-4V5-APEX2. Marker for lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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