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pGEX CblB-SH2

Plasmid

#46413

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

CblB (CBLB Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 42-359

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF770

Plasmid

#88562

Purpose

Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF770 (ZNF770 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-rPKM2 WT

Plasmid

#107158

Purpose

MBP-rPKM2 WT for production and purification of rat PKM2 WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(IRSp53 pLxIS)

Plasmid

#221315

Purpose

Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF768

Plasmid

#88524

Purpose

Donor Vector containing ZNF768 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF768 (ZNF768 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgRB1#2

Plasmid

#174145

Purpose

Lentiviral vector expressing a sgRNA against the human RB1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DOC2B_USP43

Plasmid

#205806

Purpose

Express mEGFP-tagged fusion protein, DOC2B_USP43 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DOC2B_USP43 (DOC2B, USP43 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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MigR1-AEtr

Plasmid

#12432

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

C-term truncated AML1-ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4-His-Max-A-PQBP1

Plasmid

#25802

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

His and Xpress

Expression

Mammalian

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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BAF-S4A-tet-puro

Plasmid

#199649

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Mutation

S4A

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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BAF-S4D-tet-puro

Plasmid

#199650

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Mutation

S4D

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAVA-LGF2(fs)-Gal11p(fs)

Plasmid

#127483

Purpose

Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)

Expression

Bacterial

Mutation

One nucleotide inserted between lambda CI and LGF…

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Btk-SH2

Plasmid

#46412

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Btk (BTK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 271-385

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLVCT-Dnd1-EGFP

Plasmid

#106343

Purpose

Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1-EGFP (Dnd1 Synthetic, Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd6

Plasmid

#102868

Purpose

Expresses mouse Fzd6 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd1

Plasmid

#102864

Purpose

Expresses mouse Fzd1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 1 (Fzd1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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CHAF1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176969

Purpose

Mammalian expression vector encoding CHAF1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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LRG/Rosa26

Plasmid

#105510

Purpose

Lentiviral expression of Rosa26 targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

Lentiviral

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only