177,604 results
-
Plasmid#160398PurposeBinary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert35S::vitis miR396-resistant GRF4-GIF1 chimera
UseBinary vectorAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
Tags6xHisExpressionBacterialPromoteraraBAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAD21 (RAD21 Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
ExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-mTagBFP2
Plasmid#161790Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; BicistronicTagsmTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCherry
Plasmid#155222PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
TagsmCherryExpressionMammalianAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-FOXP1
Plasmid#153145PurposeExpression of Flag tagged human FOXP1 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLEX307-APN-puro
Plasmid#158454PurposeConstitutive lentiviral expression of human APN.DepositorAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
ExpressionBacterialPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαq-LgB97 (GNAQ Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAIMTOR A757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M3-M14-dCas9
Plasmid#134779PurposeExpression of fusion protein M3-M14-dCas9 in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-AT
Plasmid#133306Purpose"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisationDepositorInsertaxe/txe toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE Gria1-GFP KI
Plasmid#131489PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 acGFP
Plasmid#128047PurposeMammalian expression vector with N terminal AcGFPDepositorTypeEmpty backboneTagsAcGFPExpressionMammalianAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only