171,035 results

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-smURFP-RBS-HO-1

Plasmid

#80341

Purpose

Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

Plasmid

#141391

Purpose

Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-N (N SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHtrA-TEV-His12

Plasmid

#137036

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Antares2 c-myc

Plasmid

#100027

Purpose

A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

Antares2

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-hBAF60b

Plasmid

#21035

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60b (SMARCD2 Human)

Use

Phagemid

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

Plasmid

#66789

Purpose

Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

D842V (constitutively active)

Promoter

EF1a

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only
pNL [NlucP/p53-RE/Hygro] Vector

Plasmid

#236920

Purpose

Express p53 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

p53 (TP53 Human)

Use

Luciferase

Tags

NanoLuc (R)

Mutation

None

Promoter

minP

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMH-MYC

Plasmid

#101765

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1c-IRES-mCherry

Plasmid

#131825

Purpose

Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC19A1

Plasmid

#132266

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Tags

5HT6 and mCherry

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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lenti SYN-FLAG-dCas9-VPR

Plasmid

#114196

Purpose

Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SYN

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Puro DEST (694-6)

Plasmid

#17297

Purpose

3rd gen lentiviral promoter-less Gateway destination vector, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCDH-Neo/Myc PD-L1

Plasmid

#121477

Purpose

expression of human Myc-PD-L1 in mamalian cell

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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hU6-pegRNA-EFS-mScarlet Template

Plasmid

#196037

Purpose

The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

Depositor

Tyler Jacks

Article

Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

Insert

mScarlet

Expression

Mammalian

Promoter

hU6 and EFS

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R]

Plasmid

#177502

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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