We narrowed to 19,295 results for: CAL
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Plasmid#170341PurposeGFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertGFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1
Plasmid#129053Purposetargeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDRDepositorInsertdCas9-huTET1CD, SgRNA1 (mouseTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8
Plasmid#129036Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA8 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1
Plasmid#129029Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA1 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
Plasmid#139482PurposeExpresses PhyB621-mCherry-HRasCT in mammalian cells.DepositorInsertPhyB621 (PHYB Mustard Weed)
UseLentiviralTagsmCherry-HRasCTExpressionMammalianMutationG229L and deletion of aa Y at position 235 in mch…PromoterTet responsible elementAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry
Plasmid#122959PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xmyc tagMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP
Plasmid#122956PurposeLentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta C terminus (NOTCH2NLB Human)
UseLentiviralTags6xhis tagMutationdeletion of C terminus from full length NOTCH2NLBPromoterCAG promoterAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500
Plasmid#92316PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92228PurposeExpression of zmADH using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500
Plasmid#92317PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable SinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-msGC-NT21
Plasmid#78102Purposeexpresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and betaDepositorInsertsfragment of alfa subunit of sGC
fragment of beta subunit of sGC
TagsHis-tagExpressionBacterialMutationfragment 1-380 of the beta subunit and fragment 2…PromoterT7Available SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Drosha270-1374S300/302A
Plasmid#62533PurposeN-terminal aa1-269 of Drosha is deleted and serines 300 &302 are mutated to alanineDepositorInserttruncated human Drosha mutant (DROSHA Human)
TagsGFPExpressionMammalianMutationdeleted amino acids 1-269 and changed serines 300…Available SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MIII
Plasmid#58683PurposeExpresses LGP2 with a mutation to motif III in mammalian cellsDepositorInsertLGP2-MIII (DHX58 Human)
Tags3x FLAGExpressionMammalianMutationthreonine and serine of helicase motif III mutate…PromoterCMVAvailable SinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
TagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a E11A/R32A
Plasmid#40055DepositorInsertSlp2-a E11A/R32A (Sytl2 Mouse)
TagsEGFPExpressionMammalianMutationGlutamate 11 to Alanine, Arginine 32 to AlaninePromoterCMV promoterAvailable SinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2-delta-N
Plasmid#27476DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeletion of coding nucleotides from 1-351. This r…Available SinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-miR-Report + MT miR-181 BS in CDX2 3UTR
Plasmid#22793DepositorInsertMutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)
UseLuciferaseAvailable SinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D444-630
Plasmid#19840DepositorInsertMKL1 D444-630 (MRTFA Human)
Tags3xFLAGExpressionMammalianMutationdeleted amino acids 444-630Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only