We narrowed to 12,928 results for: LIC

1. pAAV.GFAP.(Cyto)iGlucoSnFR

   Plasmid

   #164513

   Purpose

   Green glucose sensor, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCoofy57

   Plasmid

   #122009

   Purpose

   Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His8 and Multi1: depending on the LP2 primer, eit…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IMS-APEX2

   Plasmid

   #79058

   Purpose

   Expresses APEX in the mitochondrial intermembrane space.

   Depositor

   Alice Ting

   Article

   Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

   Insert

   APEX
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cav-GA

   Plasmid

   #209880

   Purpose

   Dimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP A
   

   Use

   Tags

   CAVIN1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209724

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPJ23116-MS2-rep

   Plasmid

   #184842

   Purpose

   Expression of the rep protein of phage MS2

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Replicase gene MS2 phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23116

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG sHRPa-NRX

   Plasmid

   #73143

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Tags

   2x HA tag and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-Cer-NCreIntN

    Plasmid

    #160505

    Purpose

    Expresses NCreIntN, tagged with HA, in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCgCas9_recT

    Plasmid

    #153337

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniTurbo-V5_pRS415

    Plasmid

    #107168

    Purpose

    expresses V5-tagged miniTurbo in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TBG-Cyto-TurboID

    Plasmid

    #149414

    Purpose

    Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    Cyto-TurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mutation

    Promoter

    TBG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM253 - sgRNA mosTI hygroR

    Plasmid

    #159827

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM270 - MCS mosTI hygroR target

    Plasmid

    #159826

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI hygroR target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FKBP-NTEVp(WT)

    Plasmid

    #137831

    Purpose

    Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-NTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB-CTEVp(WT)

    Plasmid

    #137832

    Purpose

    Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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