165,307 results

1. pBAD-GINKO2

   Plasmid

   #177116

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

   Depositor

   Robert Campbell

   Article

   Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

   Insert

   GINKO2
   

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG-CMV-hErk1

   Plasmid

   #49328

   Purpose

   Expresses FLAG-tagged human Erk1

   Depositor

   Melanie Cobb

   Insert

   Erk1 (MAPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

   Plasmid

   #138460

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-KRAB-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCM-CLYBL-NGN2-BRN3A

   Plasmid

   #165597

   Purpose

   Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.

   Depositor

   Carsten Bönnemann

   Article

   Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   BRN3A (POU4F1 Human)
   EGFP
   Puromycin N-acetyl transferase
   rtTA3G
   SBP Delta-LNGFR
   

   Use

   CRISPR and TALEN

   Expression

   Mammalian

   Promoter

   CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pemiRFP670-N1

   Plasmid

   #136556

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP670
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_GP64

   Plasmid

   #207276

   Purpose

   Mammalian expression of baculovirus GP64 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   GP64
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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