4,179 results

1. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-luciferase-P2A-Neo

   Plasmid

   #105621

   Purpose

   3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDM2p-Mdm2-YFP

   Plasmid

   #53962

   Purpose

   Expresses YFP tagged Mdm2 from MDM2 promoter

   Depositor

   Uri Alon, Galit Lahav

   Article

   Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

   Insert

   MDM2 (MDM2 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   MDM2

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mGFP-Actin

   Plasmid

   #21948

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin (ACTB Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-ChR2-Venus

   Plasmid

   #15753

   Purpose

   Expresses ChR2-Venus for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

   Insert

   Channelrhodopsin2-Venus
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPK3079

   Plasmid

   #78741

   Purpose

   Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   AsCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV CEPIA4mt

   Plasmid

   #58220

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA4mt
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L F395W D436E

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMW#3

   Plasmid

   #13350

   Depositor

   Marian Walhout

   Article

   Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

   Insert

   LacZ reporter
   

   Expression

   Yeast

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-GFP-IKBalpha-wt

    Plasmid

    #15263

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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