Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1711 - 1740 of 6032 results

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Hy_pMT eGFP-RVFV NSs HhRz

Plasmid

#91801

Purpose

Expresses a non-polyadenylated, translatable EGFP mRNA in Drosophila

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2deltPYD-T7

Plasmid

#51539

Purpose

Express T7-tagged HIN domain of hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

deletion of pyrin domain

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCW

Plasmid

#190633

Purpose

Mammalian expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SKW

Plasmid

#190631

Purpose

Bacterial expression of genetically encoded potassium indicator SKW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SKW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SCW

Plasmid

#190626

Purpose

Bacterial expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-s34T

Plasmid

#28256

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain H3-H4 with Thrombin site

Use

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between H3 and H4

Promoter

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
CS2-GFP-Ben

Plasmid

#47966

Purpose

Expression of fly Ben

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

Bendless (ben Fly)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mAIM2-PYD-EGFP

Plasmid

#51610

Purpose

Express GFP-tagged PYD of mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of HIN domain

Promoter

MESV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD-GST

Plasmid

#51534

Purpose

Express GST-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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KA1130_pPB-TREtight-cHA-IRESH2BmCherry

Plasmid

#124173

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-DH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pFPL-Yg

Plasmid

#61655

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pY128

Plasmid

#127713

Purpose

Level 1 yeast shuttle vector for a transcription unit with A - D scars. Clone in with BsaI. Has a high-copy yeast replicon and KanMX selection.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Tags

Expression

Mutation

Promoter

dU6-3

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP-Z-Cav1-His6

Plasmid

#223701

Purpose

To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP-Z-Cav1-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL854

Plasmid

#214254

Purpose

Expression eTd-CBE in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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delCMV-mCitrine

Plasmid

#214144

Purpose

Volume marker / Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCitrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_W267N

Plasmid

#214314

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

W267N

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_R269D

Plasmid

#214313

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

R269D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A

Plasmid

#195707

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B

Plasmid

#195708

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB

Plasmid

#195709

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge