We narrowed to 6,962 results for: tet on

1. hMLKL NB(1-182)-2xFv-flag

   Plasmid

   #106075

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-182)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-182

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5 FRT TO Myc ArA WT

   Plasmid

   #59804

   Purpose

   Allows the integration of Myc ArA in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5 FRT TO Myc ArA K162R

   Plasmid

   #59805

   Purpose

   Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K162R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-TRE-Gluc-RMA-IRES-EGFP

   Plasmid

   #189628

   Purpose

   Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and TRE

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRMPVneo.Yap.3093

   Plasmid

   #59915

   Purpose

   Tet on shRNA for conditional Yap1 knockdown

   Depositor

   Tyler Jacks

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hMLKL NB(1-140)-2xFv-Venus

   Plasmid

   #106076

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AXL KD

   Plasmid

   #105934

   Purpose

   Mammalian expression of myc-his tagged AXL kinase dead (K558R) mutant

   Depositor

   Rosa Marina Melillo

   Article

   Avilla et al Cancer Res. 2011 Mar 1;71(5):1792-804. d

   Insert

   AXL (AXL Human)
   

   Use

   Tags

   His and myc

   Expression

   Mammalian

   Mutation

   K558R, kinase dead

   Promoter

   tet-inducible CMV

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB-hCMV1-hKLF2-IV

   Plasmid

   #60433

   Purpose

   tet-inducible human KLF4

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   KLF2 (KLF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTER E2F1shRNA human

   Plasmid

   #66883

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F1

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 1 (E2F1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pB-MultiplexedReporter-MOR170-1

    Plasmid

    #129461

    Purpose

    piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

    Insert

    MOR170-1 (Olfr895 Mouse)
    

    Use

    Synthetic Biology

    Tags

    LUCY tag, Rho tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (Tet-On)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTER RB1shRNA human

    Plasmid

    #66886

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

    Insert

    retinoblastoma 1 (RB1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5 FRT TO Myc ArB WT RNAiR

    Plasmid

    #59806

    Purpose

    Allows the integration of Myc ArB in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    RNAi resistant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCWXPGR-pTF-Wnt3a

    Plasmid

    #114287

    Purpose

    TET-inducible expression of Wnt3a

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT TO Myc ArC Long WT

    Plasmid

    #59809

    Purpose

    Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTER E2F2shRNA human

    Plasmid

    #66884

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 2 (E2F2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

17. CIAR_pcDNA5/FRT/TO

    Plasmid

    #86502

    Purpose

    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CIAR (SOS1 Human)
    

    Use

    Frt

    Tags

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV (tet operator)

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid

    #82032

    Purpose

    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCWXPGR-pTF-DVL2deltaDIX

    Plasmid

    #114276

    Purpose

    TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    DVL2 (Dvl2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of DIX domain

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN-G1TGt

    Plasmid

    #44508

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pTER E2F3shRNA human

    Plasmid

    #66885

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 3 (E2F3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid

    #82031

    Purpose

    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Non phosphorylatable mutations on 3 of the main A…

    Promoter

    CMV-tet

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDN-G1TCbt

    Plasmid

    #44515

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRevTRE SGFP2 Inpp5E WT shRNA resistant

    Plasmid

    #161997

    Purpose

    Tet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5E (INPP5E Human)
    

    Use

    Retroviral

    Tags

    SGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5 TO FRT Myc ArB K106R

    Plasmid

    #59807

    Purpose

    Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K106R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

    Plasmid

    #139482

    Purpose

    Expresses PhyB621-mCherry-HRasCT in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    PhyB621 (PHYB Mustard Weed)
    

    Use

    Lentiviral

    Tags

    mCherry-HRasCT

    Expression

    Mammalian

    Mutation

    G229L and deletion of aa Y at position 235 in mch…

    Promoter

    Tet responsible element

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLIK-Venus-TmiR-G13

    Plasmid

    #25741

    Purpose

    Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid

    #112712

    Purpose

    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    EGFP
    

    Use

    Tags

    HIV Rev nuclear localization signal (NES), biotin…

    Expression

    Mammalian

    Mutation

    Nucleotide sequence optimized for synthetic gene …

    Promoter

    CMV with Tet repressor binding sites

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFRT-TODestGFP_RBPMS

    Plasmid

    #65744

    Purpose

    expresses GFP-tagged RBPMS in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5 FRT TO Myc ArC K72R

    Plasmid

    #59811

    Purpose

    Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K72R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFRT-TODestRFP_RBPMS

    Plasmid

    #65745

    Purpose

    expresses RFP-tagged RBPMS in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pMuLE ENTR CMV/TO-miR-30 L1-R5

    Plasmid

    #62122

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMuLE ENTR CMV/TO-miR-30 L5-L2

    Plasmid

    #62123

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMuLE ENTR CMV/TO-miR-30 L1-L4

    Plasmid

    #62124

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMuLE ENTR CMV/TO-miR-30 R4-R3

    Plasmid

    #62125

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMuLE ENTR CMV/TO-miR-30 L5-L4

    Plasmid

    #62126

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFRT-TODestFLAGHA_RBPMS2

    Plasmid

    #59390

    Purpose

    Destination vector with RBPMS2 for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

    Plasmid

    #236278

    Purpose

    Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

    Depositor

    Arnab Mukherjee

    Article

    Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

    Insert

    human aquaporin 1 (AQP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag and fkbp12

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtight

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pInducer20 2xHA GPR153

    Plasmid

    #226812

    Purpose

    Tet-inducible lentiviral vector for 2xHA GPR153 expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR153 (GPR153 Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_997253.2

    Promoter

    Available since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

    Plasmid

    #229807

    Purpose

    A CAGGS driven tet-on transactivator with an nls-EGFP reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    rtTA-IRES-nls-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pInducer20 2xHA LTB4R

    Plasmid

    #226810

    Purpose

    Tet-inducible lentiviral vector for 2xHA LTB4R expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_858043.1

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pInducer20 2xHA GPR137

    Plasmid

    #226813

    Purpose

    Tet-inducible lentiviral vector for 2xHA GPR137 expression

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Lentiviral

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_001164351

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. RNF149 shRNA-TRE

    Plasmid

    #225337

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    RNF149 shRNA (Rnf149 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (tetracycline-responsive element)

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Luciferase shRNA-TRE

    Plasmid

    #225339

    Purpose

    shRNAmir backbone under tet-operator for RNA interference, with YFP

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    Luciferase shRNA (LOC116160065 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE (tetracycline-responsive element)

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLV_TRE3G_hRTN3E-Halo

    Plasmid

    #216252

    Purpose

    Tet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vector

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon-3 isoform e (RTN3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pKT-CNP-inact

    Plasmid

    #211804

    Purpose

    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H73L H153L

    Promoter

    tet

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pKT-CNP

    Plasmid

    #211803

    Purpose

    Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tet

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge