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pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

Plasmid

#111393

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
GCaMP6f

Use

AAV

Tags

6xHis, Myc, T7, and Xpress

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Please see Depositor Comments

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40

Plasmid

#194689

Purpose

hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

Plasmid

#194697

Purpose

HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

elavl3(HuC)

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

Plasmid

#194698

Purpose

HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

elavl3(HuC)

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_04

Plasmid

#194665

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_04 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_06

Plasmid

#194667

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_06 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_07

Plasmid

#194668

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_07 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_07

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_08

Plasmid

#194669

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_08 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_08

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-Caprola_10

Plasmid

#194671

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_10 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_10

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-Caprola_11

Plasmid

#194672

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_11 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_11

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-Caprola_12

Plasmid

#194673

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_12 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_12

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-Caprola_02

Plasmid

#194663

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_02 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_02

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-51b(+)-Caprola_03

Plasmid

#194664

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_03 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_03

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.1 R57A, R59A pMT2

Plasmid

#206112

Purpose

expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

Depositor

Annette Dolphin

Article

Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

Insert

cacna1a (Cacna1a Rat)

Use

Tags

Expression

Mammalian

Mutation

R57A, R59A

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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