We narrowed to 2,389 results for: dap

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Clathrin-LCa-EYFP

Plasmid

#21741

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pET24a-AlkB deltaN11

Plasmid

#73622

Purpose

Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

Depositor

Catherine Drennan

Article

Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

AlkB (alkB E. coli)

Tags

His

Expression

Bacterial

Mutation

deletion of first 11 AA

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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EcNusGFL

Plasmid

#113122

Purpose

Expresses full-length E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG (nusG Escherichia coli)

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CrkII

Plasmid

#50731

Purpose

mammalian expression of CrkII

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII (CRK Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBMN-HA-NAP1

Plasmid

#208868

Purpose

For mammalian expression of NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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AIRAP 9: PSMD2.GST.V2.pcDNA3

Plasmid

#21799

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD2 (PSMD2 Human)

Tags

GST

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSV-PGC1

Plasmid

#3

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Expression

Mammalian

Available Since

May 23, 2006

Availability

Academic Institutions and Nonprofits only
pYX233

Plasmid

#163762

Purpose

Empty vector for Gal-inducible yeast expression

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Type

Empty backbone

Tags

Hemagglutinin (HA) tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

HA

Expression

Mammalian

Promoter

Tet-responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ823

Plasmid

#39313

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (lin2744 Listeria innocua)

Use

CRISPR

Tags

MBP and thioredoxin-His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-TRAF2 (GST)

Plasmid

#21586

Depositor

John Kyriakis

Article

Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Tags

GST

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
NavBp

Plasmid

#100014

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NavBp

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Clathrin-LCa-ECFP

Plasmid

#21742

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only