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pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-∆ARD_Flag2-Blast

Plasmid

#175041

Purpose

Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein with deletion of the a…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERGIC1__RANBP17

Plasmid

#116816

Purpose

Lentiviral expression of ERGIC1__RANBP17 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERGIC1__RANBP17

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Gateway Cloning

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp2-SH2

Plasmid

#46460

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp2 (BCAR3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 144-259

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P5-6D

Plasmid

#51254

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Asp

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120294

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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HOXA10 (human) bluescript

Plasmid

#8617

Depositor

Corey Largman

Article

Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

Insert

HOXA10 (HOXA10 Human)

Expression

Bacterial

Mutation

improved Ribosomal binding site (NcoI site) at AT…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pCW57.1 tTA SDHA

Plasmid

#177976

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

SDHA (SDHA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Silent point mutations to SDHA sgRNA target

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-MCM5__LYN

Plasmid

#116828

Purpose

Lentiviral expression of MCM5__LYN fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MCM5__LYN

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

Plasmid

#113040

Purpose

AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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WFS1 mRFP-N2 (891)

Plasmid

#62018

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGEX chimerin2-SH2

Plasmid

#46416

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin2 (CHN2 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 49-155

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-WNK1__PRMT8

Plasmid

#116845

Purpose

Lentiviral expression of WNK1__PRMT8 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WNK1__PRMT8

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pCMV

Plasmid

#8815

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Grb2-SH2 RK

Plasmid

#46441

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 RK (GRB2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HIS-tag pET new RBS

Plasmid

#8525

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Contains improved Ribosomal binding site; makes …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Expression

Mammalian

Promoter

CBh and human U6

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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