165,775 results

1. pLIB-FLAG(N)-RPB1

   Plasmid

   #231722

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   Flag-3C

   Expression

   Insect

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pminiTol2

   Plasmid

   #31829

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Egr1

   Plasmid

   #11729

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   Egr-1 (Egr1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_221

   Plasmid

   #169142

   Purpose

   Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

   Depositor

   John Doench, David Root

   Insert

   EGFP , EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

   Plasmid

   #220090

   Purpose

   Recruitable PIP5K to make PIP2

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   PIP5K1C (PIP5K1C Human)
   

   Tags

   TagBFP-FKBP1A(3-108)

   Expression

   Mammalian

   Mutation

   amino acids 1-635; D101R, R304D, R445E, K446E

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet3-alphaTubulin_C1

   Plasmid

   #189768

   Purpose

   Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-alphaTubulin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. iC13 PB-TO-oNgn2-BFP-puro

   Plasmid

   #204733

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Optimized neurogenin-2
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-NSP4SA11

   Plasmid

   #89171

   Purpose

   Express Rotavirus SA11 NSP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP4
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQCXIP mScarlet-MOSPD2 WT

    Plasmid

    #186472

    Purpose

    Expression of human MOSPD2 fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAST-attB-yki:V5-S111A, S168A, S250A

    Plasmid

    #85620

    Purpose

    Expresses V5 tagged triple mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S111A,S168,S250A
    

    Tags

    V5

    Expression

    Insect

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mito-V5-BS2 (Mitochondrial matrix)

    Plasmid

    #220344

    Purpose

    Mammalian expression vector BS2 in mitocondria

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    MTS-matrix and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. V5-BS2-SENP2 (Nuclear Pore)

    Plasmid

    #220345

    Purpose

    Mammalian expression vector BS2 in nuclear pore

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    SENP2 (nuclear pore localized protein) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pHAGE-EFS-foldon-GFP- PCP

    Plasmid

    #194503

    Purpose

    Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Insert

    foldon-GFP-PCP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. opto-Cdc42

    Plasmid

    #174510

    Purpose

    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Cdc42-mCherry
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-ERK2

    Plasmid

    #118296

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.4_17G6-1 heavy chain

    Plasmid

    #198249

    Purpose

    recombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    heavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
    

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. negMaLionB

    Plasmid

    #113911

    Purpose

    negative control for Blue ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionB
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPkan-S

    Plasmid

    #41017

    Purpose

    PCR template for en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    Kana_I-SceI
    

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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