162,325 results

1. pSP64-mHCN2

   Plasmid

   #53060

   Purpose

   Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

   Insert

   Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   E55G, R237H, and K283R polymorphisms compared to …

   Promoter

   SP6

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES proHB-EGF WT

   Plasmid

   #11602

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pLenti CMV/TO Hygro DEST (693-2)

   Plasmid

   #17291

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-3xFLAG-Blast

   Plasmid

   #207717

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBbS5a-OptoCre-REDMAP

   Plasmid

   #228465

   Purpose

   Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

   Depositor

   Mary Dunlop

   Article

   Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

   Insert

   OptoCre-REDMAP
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacUV5

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-19:LAMP1-mEGFP

   Plasmid

   #101782

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

   Depositor

   Allen Institute for Cell Science

   Insert

   LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcI-ts,ind+-(mtSSB delta20)

   Plasmid

   #186280

   Purpose

   Expresses Human Mitochondrial SSB in E. coli

   Depositor

   Kenneth Johnson

   Article

   Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

   Insert

   Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   First 20 amino acids corresponding to probable mi…

   Promoter

   lambda

   Available since

   July 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLVX-opto-hCaspase-8

   Plasmid

   #208786

   Purpose

   Inducibly expresses human Caspase 8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Caspase 8 (CASP8 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   CARD domain is removed

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB1-SCFP3A

    Plasmid

    #103970

    Purpose

    Plasmid encoding SCFP3A

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    SCFP3A
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG/GFP

    Plasmid

    #139980

    Purpose

    expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

    Depositor

    William Pu

    Article

    Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

    Insert

    Green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

    Plasmid

    #161580

    Purpose

    Express Kir2.1 in cells not expressing Cre.

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEYFP-N3-EPAC1

    Plasmid

    #113110

    Purpose

    Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

    Depositor

    Xiaodong Cheng

    Article

    Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

    Insert

    RapGEF3 (RAPGEF3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    PCMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD649-HAsp-SEMA4A-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156938

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA4A (SEMA4A Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB-GFP

    Plasmid

    #72203

    Purpose

    screen AB42 variants for aggregation

    Depositor

    Michael Hecht

    Article

    Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

    Insert

    AB42 (APP Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hCtRNA_FT

    Plasmid

    #186715

    Purpose

    Fragment template for DAP array

    Depositor

    Xue Gao

    Article

    Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

    Insert

    gRNA scaffold and hCtRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCtRNA

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE-OKSM

    Plasmid

    #204498

    Purpose

    Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

    Depositor

    Wataru Fujii

    Article

    Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

    Inserts

    OCT4 (POU5F1 Human)
    KLF4 (KLF4 Human)
    SOX2 (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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