159,557 results

1. pcDNA3.1-mArg1

   Plasmid

   #34573

   Depositor

   Peter Murray

   Insert

   Arg1 (Arg1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORTMAGE312B

   Plasmid

   #128969

   Purpose

   Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   xylS-Pm

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

   Plasmid

   #29654

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   10xAsn, 6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPUR-hU6-sgRNA-Sirius-8XMS2

   Plasmid

   #121942

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XMS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1-mTrim21ΔRING-Box

   Plasmid

   #105517

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   CMV

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPA-GFP-Tubulin-C-18

   Plasmid

   #57160

   Purpose

   Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. ZJOM65

   Plasmid

   #133654

   Purpose

   Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPH1-mCherry (VPH1 Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-HA-NLS-I-PpoI

   Plasmid

   #46963

   Purpose

   For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   NLS-I-PpoI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TET

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-Cys-DogCatcher

   Plasmid

   #171929

   Purpose

   Express Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   Cys-DogCatcher
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-cMSP1E3D1

    Plasmid

    #199772

    Purpose

    Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

    Insert

    cMSP1E3D1
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Extended cMSP1D1; external His-Tags flanking the …

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYES2-HTH

    Plasmid

    #86470

    Purpose

    Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Type

    Empty backbone

    Use

    Tags

    HIS10-TEV-HA

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

    Plasmid

    #90332

    Purpose

    CRISPR interference

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dCas9
    na
    SFFV
    P2A-BSD
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SELP-bio-His

    Plasmid

    #51639

    Purpose

    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SELP (SELP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_SPOP_WT

    Plasmid

    #81856

    Purpose

    Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmAmetrine-DEVD-tdTomato

    Plasmid

    #18879

    Purpose

    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

    Depositor

    Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Insert

    mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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