We narrowed to 41,529 results for: TRO

1. CDS12_mTurquoise2-linker

   Plasmid

   #136090

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mTurquoise2(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. CDS12_mVenus-linker

   Plasmid

   #136092

   Purpose

   FP for N-term fusion with a CTAG without linker

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   CDS12_mVenus(noStop)-SerGly10xAlaSer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. CDS12_TP-MpSIG2

   Plasmid

   #136093

   Purpose

   Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   TP-MpSIG2, Mapoly0214s0004
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCsA_spacer

   Plasmid

   #136075

   Purpose

   Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

   Plasmid

   #174528

   Purpose

   Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

   Depositor

   Yu-Hsuan Tsai

   Article

   Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

   Insert

   PylS
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. S111

   Plasmid

   #174549

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   BclxL and designed protein LD3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S112

   Plasmid

   #174550

   Purpose

   Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)

   Depositor

   Bruno Correia

   Article

   Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

   Insert

   Bcl2 and designed protein LD3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH191

   Plasmid

   #172149

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*, Y184*); [strong promoter eG, strong RB…

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH61

   Plasmid

   #172143

   Purpose

   Accessory Plasmid for PACE of pylBCD variants

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   gIII(P29*); [strong promoter eG, strong RBS SD8] …

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH190

    Plasmid

    #172148

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Promoter

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJC8

    Plasmid

    #170757

    Purpose

    Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.

    Depositor

    Roberto Dominguez

    Article

    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

    Type

    Empty backbone

    Use

    Tags

    site 1- V5 tag and site 2- FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTJV1ScGm-rpoB

    Plasmid

    #166979

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    gentamycin-3-acetyltransferase (aac(3)-Ia )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. C415-E01: attB4-stuffer-attB1r

    Plasmid

    #162931

    Purpose

    Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS405 VAC8-G2A-GFP

    Plasmid

    #166837

    Purpose

    Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    G2A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLJM1-zeo-2xStrep-ORF68

    Plasmid

    #162659

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-zeo-2xStrep-UL52

    Plasmid

    #162663

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged UL52 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL52
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)

    Plasmid

    #159888

    Purpose

    Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    [4-1] - Promoter - Pmlc-1 (w ATG)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)

    Plasmid

    #159889

    Purpose

    Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    [4-1] - Promoter - Pmlc-2(no_start)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EcNusGKOW

    Plasmid

    #113124

    Purpose

    Expresses C-terminus of E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-124

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EcRfaHKOW

    Plasmid

    #113125

    Purpose

    Expresses C-terminus of E. coli RfaH

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107

    Promoter

    T7

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge