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RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

Plasmid

#145842

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-G185R

Plasmid

#125849

Purpose

express mutant BAP1-G185R

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

G185R

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-MaoB

Plasmid

#100757

Purpose

Mammalian expression of mitochondria targetted Nix

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-MaoB (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Mutation

TM domain replaced by MaoB

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC-S227A

Plasmid

#200560

Purpose

Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.CD86.C-HA

Plasmid

#154848

Purpose

Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Cluster of Differentiation 86 (Cd86 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
LRG/Rosa26

Plasmid

#105510

Purpose

Lentiviral expression of Rosa26 targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

Lentiviral

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-E31A

Plasmid

#125843

Purpose

express mutant BAP1-E31A

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

E31A

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-S10T

Plasmid

#125842

Purpose

express mutant BAP1-S10T

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

S10T

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)3x

Plasmid

#112057

Purpose

ACTB mRNA tagged with 3 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

3 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)2x

Plasmid

#112056

Purpose

ACTB mRNA tagged with 2 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

2 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-257

Plasmid

#31063

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-257 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-L49V

Plasmid

#125844

Purpose

express mutant BAP1-L49V

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

L49V

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-164

Plasmid

#31066

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-164 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a C2AB

Plasmid

#40051

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a C2AB (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2AB domains only (aa 597-910)

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-EglN2 H358A-pBabe-puro

Plasmid

#22705

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated cDNA: point mutation in codon 358 changi…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II tension sensor (FL-based)

Plasmid

#118721

Purpose

The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

inserted FL-based tension sensor module after aa1…

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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