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pMT-GFP-Pat coiled-coil (cc)

Plasmid

#31757

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Insect

Mutation

Contains only the coiled-coil domain: aa 535-1457

Promoter

Metallothionein

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pGbH500-Nanog-AXP

Plasmid

#140279

Purpose

a targeting plasmid for Nanog-APEX

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2

Use

Mouse Targeting

Promoter

endogenous

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-2xGfaABC1D-tTA

Plasmid

#19981

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xGfaABC1D-tTA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only

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RhoH

Plasmid

#55697

Purpose

expression of mouse RhoH

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoH (Rhoh Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-29

Plasmid

#120008

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K19R

Plasmid

#44749

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
deltaCT S6K1 MSCV puro

Plasmid

#8465

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta ct p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pBS cFgfr3 (CT#219)

Plasmid

#14004

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Fgfr3 (FGFR3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
p5xUAS

Plasmid

#165891

Purpose

Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xUAS

Expression

Insect

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p5xQUAS

Plasmid

#165818

Purpose

Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xQUAS

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV myc DAF-12

Plasmid

#32566

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12 (daf-12 Nematode)

Tags

myc epitope

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pMIR-R/Tac1/SG

Plasmid

#16296

Depositor

Pranela Rameshwar

Article

Greco et al Proc Natl Acad Sci U S A. 2007 Sep 25. 1

Insert

Tac1 3'UTR (TAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pOBCol3.6-MCS

Plasmid

#108311

Purpose

Drive inserted gene in type 1 collagen expressing cells.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2

Plasmid

#111698

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hisA cdc25c C330S C377S

Plasmid

#10965

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

C330S C377S. Cysteine to Serine substitution at …

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pJUMP39-1A(sfGFP)

Plasmid

#126983

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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MLM3587

Plasmid

#43886

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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MLM2585

Plasmid

#43890

Purpose

Activation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NN (binds G)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator p65 backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only