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pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBS Dynein light chain 1

Plasmid

#19213

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Dynein light chain 1

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
GST-RNF126 C231A

Plasmid

#138644

Purpose

Bacterial expression of GST-RNF126 RING mutant (C231A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C231A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-RASSF1C

Plasmid

#1977

Depositor

Joseph Avruch

Article

Ortiz-Vega et al Oncogene 2002 Feb 21;21(9):1381-90.

Insert

RASSF1C (Rassf1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
p413GPD-hTPI Ile170Val

Plasmid

#50720

Purpose

expression of the human TPI Ile170Val allele in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Mutation

Isoleucine 170 to Valine

Promoter

GPD

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S129N

Plasmid

#51587

Purpose

expresses RNAiR myc-tagged Rem2 non-GTP binding

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

S129N; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 L317G

Plasmid

#51586

Purpose

expresses RNAiR myc-tagged Rem2 non-CaM interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

L317G; silent mutations at shRNA 1 and 2 recognit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_C

Plasmid

#127036

Purpose

Basic Part C- C-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSuperRetro mouse Hic-5

Plasmid

#11187

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (Tgfb1i1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pT2MP.EF-Q2m

Plasmid

#129310

Purpose

QUEEN ATP sensor for 25degC, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Axin DeltaGID/pCS2MT

Plasmid

#16303

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin DeltaGID (axin1.L Frog)

Use

Vertebrate expression

Tags

5x myc

Mutation

Delta GID: deletion of GSK interaction domain aa …

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCH043

Plasmid

#47949

Purpose

rtTA under the control of GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCH044

Plasmid

#47950

Purpose

rtTA under the control of GAL2 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal2

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMH184

Plasmid

#122857

Purpose

MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

MS2-modified sgRNA targeting the Arabidopsis FT promoter

Use

Golden gate compatible cloning vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJB073

Plasmid

#98912

Purpose

Express Dronpa tagged ZapB in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB

Tags

Dronpa

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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FUGW-H1-Syndecan-1 shRNA (UTR)

Plasmid

#40623

Depositor

Sally Temple

Article

Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

Insert

Syndecan-1 shRNA(UTR) (Sdc1 Mouse)

Use

Lentiviral and RNAi

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only