We narrowed to 7,235 results for: ALP

1. pSpCas9n(BB)-2A-puro-a5 guide B

   Plasmid

   #194011

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpCas9n(BB)-2A-puro-aV guide A

   Plasmid

   #194008

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9n(BB)-2A-puro-aV guide B

   Plasmid

   #194009

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpCas9n(BB)-2A-puro-a5 guide A

   Plasmid

   #194010

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Integrin-αv Headpiece M400GC

   Plasmid

   #159637

   Purpose

   Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   alpha v headpiece M400GC
   

   Use

   Tags

   6x His and Strep II

   Expression

   Mammalian

   Mutation

   M400C

   Promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L304-EGFP-Tubulin-K40R

   Plasmid

   #64059

   Purpose

   EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

   Insert

   tubulin
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pNL(CMV)GLuc/CMV/WPREdU3

   Plasmid

   #136999

   Purpose

   Reporter vector encoding Gaussia luciferase

   Depositor

   Jakob Reiser

   Article

   Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

   Insert

   Gaussia luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGFP Glra2 (CC#301)

   Plasmid

   #15199

   Depositor

   Connie Cepko

   Article

   Young et al Neuron. 2004 Mar 25. 41(6):867-79.

   Insert

   Glra2 (GLRA2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-miR-29b-1~29a

   Plasmid

   #51376

   Depositor

   Narry Kim

   Article

   Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

   Insert

   pri-miR-29b-1~29a (MIR29B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCEF-VSV-G

    Plasmid

    #41792

    Purpose

    VSV-G-expressing envelope plasmid

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Vesicular stomatitis virus G glycoprotein, Indiana strain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CEF

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1.2-Neo

    Plasmid

    #162739

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p1.2-Hygro

    Plasmid

    #162741

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG416GPD-Cerulean-GOS1

    Plasmid

    #18846

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    GOS1 (GOS1 Budding Yeast)
    

    Use

    Tags

    cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBB14

    Plasmid

    #18657

    Depositor

    Lynn Enquist

    Article

    Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

    Insert

    Us9-EGFP gene fusion
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First 106 amino acids of Us9 are intact. The stop…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C413-E15: attB4-EF1p>-attB1r

    Plasmid

    #162920

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human EF1alpha promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-pOTC-3H-GFP

    Plasmid

    #205725

    Purpose

    Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    pOTC-3H-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416GPD-Cerulean-SEC4

    Plasmid

    #18844

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    SEC4 (SEC4 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

18. CFP_Rad_pcDNA4_HisMaxC

    Plasmid

    #41654

    Depositor

    Henry Colecraft

    Article

    Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

    Insert

    Rad, GTP binding proteins (RGK) (Rrad Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS426-TUB1

    Plasmid

    #60385

    Purpose

    Expression of yeast alpha tubulin (TUB1) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pREP3-TEV-NLS

    Plasmid

    #21146

    Depositor

    Stephen Kearsey

    Article

    Yang et al BMC Mol Biol. 2005 . 6():13.

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Use

    S. pombe

    Tags

    2xNLS, NLS, and myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQUAST-syb-spCFP1-10

    Plasmid

    #73699

    Purpose

    Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spCFP1–10 chimera
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. cacna2d1 R241A D491A HA pcDNA3

    Plasmid

    #206109

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R241A D491A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KG#65

    Plasmid

    #110933

    Purpose

    Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

    Inserts

    unc-17 beta promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p70 delta2-46 delta CT104

    Plasmid

    #1898

    Depositor

    Joseph Avruch

    Article

    Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

    Insert

    p70 alpha1 S6 kinase (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta2-46, deltaCT104 (deletion of aa 2-46 and am…

    Promoter

    Available since

    March 17, 2006

    Availability

    Academic Institutions and Nonprofits only

25. KG#45

    Plasmid

    #110926

    Purpose

    Vector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegans

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    hsp-16-2 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGFP Glra2 KA (CC#303)

    Plasmid

    #15201

    Depositor

    Connie Cepko

    Article

    Young et al Neuron. 2004 Mar 25. 41(6):867-79.

    Insert

    Glra2 (GLRA2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K310A

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

27. p1.2-Zeo

    Plasmid

    #162740

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. C453-E14: attB4-EF1p>-attB5

    Plasmid

    #162975

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    human EF1alpha promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. a5-ecto-pcDNA31hygro

    Plasmid

    #113386

    Purpose

    Express human alpha5 ectodomain with secretion peptide, purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

    Insert

    human alpha 5 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FR_luc

    Plasmid

    #31098

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    firefly luciferase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

31. pRS424-TUB1-Cterminal6xHis

    Plasmid

    #60387

    Purpose

    Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCellFree_G03 S100A1

    Plasmid

    #67048

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    S100A1 (S100A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pAG416GPD-Cerulean-YPT7

    Plasmid

    #18847

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT7 (YPT7 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

34. pMiniWhiteA2

    Plasmid

    #165873

    Purpose

    Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLPS-hAkt1-gfp

    Plasmid

    #49388

    Purpose

    mammalian expression of human Akt1 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    CMV

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

36. a4 ecto pcDNA3.1 hygro(-)

    Plasmid

    #113406

    Purpose

    expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

    Insert

    human alpha 4 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMiniwhiteA1

    Plasmid

    #165872

    Purpose

    Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAG416GPD-SEC7-Cerulean

    Plasmid

    #18851

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    SEC7 (SEC7 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

39. pBacPAK8-VHL

    Plasmid

    #29498

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Von Hippel-Lindau (VHL Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pRevLCav2

    Plasmid

    #60806

    Purpose

    used to make mutations in yeast REV3

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Promoter

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge