We narrowed to 6,296 results for: pcDNA 3.1

Showing: 2901 - 2950 of 6296 results

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pcDNA-Sga-Cas9

Plasmid

#227005

Purpose

Mammalian expression of Nuclease active S. gallolyticus Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sin-Cas9

Plasmid

#227006

Purpose

Mammalian expression of Nuclease active S. iniae Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Spa-Cas9

Plasmid

#227007

Purpose

Mammalian expression of Nuclease active S. parasanguinis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Sub-Cas9

Plasmid

#227008

Purpose

Mammalian expression of Nuclease active S. uberis Cas9

Depositor

Charles Gersbach

Article

Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Tags

PuRRRE

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Tags

TREER

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TinyERRE

Plasmid

#208324

Purpose

C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TinyERRE

Tags

TinyERRE

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PunyEEER

Plasmid

#208326

Purpose

C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PunyEEER

Tags

PunyEEER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-hTERT

Plasmid

#51637

Purpose

Mammalian expression of 3xHA tagged human TERT, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

TERT (TERT Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos DD

Plasmid

#8968

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-ATBF1

Plasmid

#40927

Depositor

Jin-Tang Dong

Article

Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

Insert

ATBF1 (ZFHX3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin3 (CUL3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Pcbp2

Plasmid

#184028

Purpose

Codon optimized Pcbp2 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Pcbp2 (Pcbp2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Codon optimized

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4B (CUL4B Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Rod Opsin

Plasmid

#109361

Purpose

Human rod opsin

Depositor

Robert Lucas

Article

Bailes et al Proc Biol Sci. 2013 Apr 3;280(1759):2012

Insert

Rod opsin (RHO Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-BIRC3-LU

Plasmid

#160700

Purpose

Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA_RGG-GFP-RGG

Plasmid

#124930

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available Since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-H2B-Venus

Plasmid

#87614

Purpose

Venus (YFP) fused to histone H2B.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

H2B-Venus (H2BC8 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Mirk (pHX9F)

Plasmid

#17846

Depositor

Eileen Friedman

Article

Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

Insert

Mirk (DYRK1B Human)

Tags

6xHis and Anti-XPress

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-BIRC3-SU

Plasmid

#160699

Purpose

Expresses GFP-BIRC3 with the short 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-ERK2 D319N

Plasmid

#8979

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

D319N

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-FLAG-Bbs4

Plasmid

#128868

Purpose

For mammalian expression of mouse Bbs4

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Bbs4 (Bbs4 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-NPY-ClopHensor

Plasmid

#25939

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)

Tags

NPY and Strep-II

Expression

Mammalian

Mutation

Neuropeptide Y localization signal

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GFP-BIRC3-NU

Plasmid

#160698

Purpose

Expresses GFP-BIRC3 without the endogenous 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Stra6L-6His

Plasmid

#174215

Purpose

Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

Depositor

Adrian Press

Article

Stafast et al ACS Appl. Polym. Mater. 2022

Insert

Stra6L (Stra6l Mouse)

Tags

6-His

Expression

Mammalian

Mutation

non

Promoter

CMV

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA_RGG-RFP-RGG

Plasmid

#124934

Purpose

Expression of RGG-RFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-SETin-FLAG-HA

Plasmid

#24999

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations introduced to prevent siRNA targ…

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-NLS-ZapCmR2

Plasmid

#59012

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR2 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

Y261A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgH (Merlin)

Plasmid

#136448

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-NLS-ZapCmR1.1

Plasmid

#59011

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR1.1 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L198A

Plasmid

#8975

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-RELΔ424-490

Plasmid

#27267

Depositor

Thomas Gilmore

Article

Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

Insert

REL (REL Human)

Expression

Mammalian

Mutation

Deletion of transactivation domain I (aa 425-490)

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Ubc13

Plasmid

#212806

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Mms2

Plasmid

#212807

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA.3-V5-SPOP (R221C)

Plasmid

#197022

Purpose

Mammalian expression vecor for V5-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R221C

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 6,296 results for: pcDNA 3.1