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pTR-473 FOXP1-tNGFR

Plasmid

#186063

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

FOXP1 (FOXP1 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pnCS_DSep2_Peanut-TEV-Strep

Plasmid

#174495

Purpose

bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

Plasmid

#134291

Purpose

Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30, 280 and 443 to Asparag…

Promoter

T7

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5-toku (1-174)

Plasmid

#134261

Purpose

Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-174

Promoter

CMV

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N)

Plasmid

#134292

Purpose

Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30 and 280 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (416-534)

Plasmid

#134267

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 416-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (396-534)

Plasmid

#134266

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 396-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 396-534

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-534)

Plasmid

#134264

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-534)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-534

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (25-287)

Plasmid

#134263

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 25-287

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (1-287)

Plasmid

#134262

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-287

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (287-396)

Plasmid

#134265

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 287-396)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 287-396

Promoter

CMV

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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mTet1 flox donor

Plasmid

#117147

Purpose

Making Tet1 conditional knock out

Depositor

Jacob Hanna

Article

Zviran et al ssrn.3155731

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_26

Plasmid

#60306

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

OBFC2A enhancer (NABP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_34

Plasmid

#60313

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PNMA2 enhancer (PNMA2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C5_12

Plasmid

#60319

Purpose

This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

Non active element (nearest TSS C18orf26)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C5_14

Plasmid

#60321

Purpose

This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

Non active element (nearest TSS CCRN4L)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_7

Plasmid

#60256

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_15

Plasmid

#60262

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

FAM148A enhancer (C2CD4A Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_17

Plasmid

#60263

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFAND3 enhancer (ZFAND3 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_18

Plasmid

#60264

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZBED3 enhancer (ZBED3 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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