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pSLIRES-11 (CC#13)

Plasmid

#15145

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Type

Empty backbone

Use

Adapter vector for cloning into prisap

Tags

IRES

Expression

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.0

Plasmid

#208301

Purpose

Expresses ABE-RA3.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA2.0

Plasmid

#208299

Purpose

Expresses ABE-RA2.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA3.2

Plasmid

#208303

Purpose

Expresses ABE-RA3.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA3.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

E27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/er-(n2)oxStayGold(c4)v2.0

Plasmid

#186296

Purpose

A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.

Depositor

Atsushi Miyawaki

Article

Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

Insert

er-(n2)oxStayGold(c4)v2.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HA-BI-AR

Plasmid

#171234

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor (AR Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-msfGFP

Plasmid

#180328

Purpose

mammalian expression of human SEPT9_i1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-msfGFP

Plasmid

#180322

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i5-msfGFP

Plasmid

#180327

Purpose

mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR

Plasmid

#28235

Purpose

Mammalian expression of Androgen receptor fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

Androgen receptor (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only

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pMK265 (RAD21-mAC Hygro)

Plasmid

#140539

Purpose

RAD21 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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mAID-BRD4 donor

Plasmid

#140650

Purpose

BRD4 tagging with mAID

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

BRD4 (BRD4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FcD265A-FLAG

Plasmid

#65420

Purpose

Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production

Depositor

Dane Wittrup

Article

Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

Insert

FcD265A-FLAG

Use

Tags

FLAG

Expression

Mammalian

Mutation

D265A

Promoter

Modified CMV Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
BRD4-N CRISPR

Plasmid

#140651

Purpose

BRD4 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

BRD4 (BRD4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone1

Plasmid

#162122

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D1-25-msfGFP

Plasmid

#180333

Purpose

mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D1-25

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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