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Plasmid#138795PurposeConstitutive expression of DsRed-Express2-ZF21 under the CMV promoter.DepositorInsertDsRed-Express2-ZF21
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorInserthuMb2Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-axon-jGCaMP8f-WPRE
Plasmid#186041PurposejGCaMP8f expression in axons under the control of the synapsin promoterDepositorInsertaxon-jGCaMP8f
UseAAVTagsGAP43-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY129 (pcDNA3.1-U6-huMb3Cpf1)
Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUA97
Plasmid#69340PurposeIt encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategyDepositorInsertceBFP SEC 3xFlag
UseTags3xFlag and ceBFP (mTagBFP)ExpressionWormMutationsqt-1(e1350)PromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXPR_016
Plasmid#107143PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8m-WPRE
Plasmid#186036PurposeAAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoterDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8s-WPRE
Plasmid#186037PurposeAAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoterDepositorInsertNES-jGCaMP8s
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::tagBFP2
Plasmid#124321PurposeExpressing Nuclear Localized tagBFP2DepositorInserttagBFP2 (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>GAL80
Plasmid#104874PurposeHACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.DepositorInsertT2A-GAL80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (ErmR)
Plasmid#177206PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL
Plasmid#192602PurposeAn AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.DepositorInsertSERCaMP
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8f-WPRE
Plasmid#186035PurposeAAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoterDepositorInsertNES-jGCaMP8f
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBac[6xP3-Tc-hsp-ECFP-SV40]
Plasmid#86447PurposepBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)DepositorInsert6xP3-Tc-hsp-ECFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
HCP5
Plasmid#166107PurposeThis plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.DepositorInsertCyr1CT-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV Tor1a-V5
Plasmid#175135PurposeLentiviral expression of Tor1a-MycDepositorInsertTor1a (Tor1a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_FLEX_tau-APEX2
Plasmid#171937PurposeExpresses APEX2 linked to tau for labeling microtubulesDepositorInserttau-APEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhsynAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only