We narrowed to 1,843 results for: rigi

1. dCas9v2

   Plasmid

   #103140

   Purpose

   TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

   Depositor

   Jens Nielsen

   Article

   Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Removed scaffold gRNA from original plasmid

   Promoter

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK1004 (pSL3)

   Plasmid

   #190999

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Brigitte Gasser, Diethard Mattanovich

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO, native, native (respectively)

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pR6Kg-Alpha1

   Plasmid

   #165862

   Purpose

   Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLQ-KO-tgt50

   Plasmid

   #126578

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOGG010

   Plasmid

   #113984

   Purpose

   Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   RK2 (IncP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL2-GGA(EAAAK)2AGG

   Plasmid

   #137104

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   GGA(EAAAK)2AGG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRMTK-clo6

   Plasmid

   #203957

   Purpose

   Cloning plasmid containing the kanamycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Kanamycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CP1012-CBEmax

    Plasmid

    #119801

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1012) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CP1028-ABEmax

    Plasmid

    #119807

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1028)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Use

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBTK1003 (pSL2)

    Plasmid

    #190998

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23110

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRMTK-clo10

    Plasmid

    #203961

    Purpose

    Cloning plasmid containing the erythromycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Erythromycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    N/A

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CP1300-ABEmax

    Plasmid

    #119810

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23107

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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