We narrowed to 8,037 results for: sms

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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STR-KDEL_PGRN-SBP-mCherry

Plasmid

#180439

Purpose

Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

Depositor

Shawn Ferguson

Publication

Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Progranulin (GRN Human)

Tags

SBP-mCherry

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A382T

Plasmid

#133756

Purpose

Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Mutation

Changed alanine 382 to threonine

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-RB (561-673)

Plasmid

#31495

Depositor

Julien Sage

Publication

Saddic et al J Biol Chem. 2010 Nov 26. 285(48):37733-

Insert

RB (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains amino acids 561-673

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Publication

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Publication

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Publication

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway Cloning

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pBS cPitx2 (CT#73)

Plasmid

#13927

Depositor

Cliff Tabin

Publication

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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p8327 pLIX YAP1 S127A

Plasmid

#184528

Purpose

Expression of YAP1 S127A

Depositor

Elizabeth White

Publication

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Lentiviral

Mutation

S127A

Promoter

TRE promoter, Tet ON

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Zhi3-mCherry

Plasmid

#106295

Purpose

CMV driven mCherry, zeocin resistance

Depositor

Sanford Simon

Publication

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DHFR.cHSF1

Plasmid

#134739

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Publication

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pScaf-1512.1

Plasmid

#111402

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-1512

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Purpose

Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSAMQ79G,Q139G-5HT3 HC

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pAC5.1-bgal-V5His

Plasmid

#136238

Purpose

Protein expression of Beta-galactosidase with C-terminal V5 and His tags

Depositor

Steven Reppert, Aziz Sancar

Publication

Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

Insert

Beta-galactosidase

Tags

His and V5

Expression

Insect

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-FLAG-Sestrin2-E451A

Plasmid

#73673

Purpose

overexpression

Depositor

David Sabatini

Publication

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pHIS3p:yomWasabi-Tub1+3'UTR::HPH

Plasmid

#50631

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

yomWasabi-Tub1

Use

Yeast expression

Tags

yomWasabi

Promoter

HIS3p

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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