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pcDNA5-F-AF9

Plasmid

#49428

Purpose

Mammalian expression of Flag-tagged human AF9

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

AF-9 (MLLT3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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Lenti_EFS-Cas9-P2A-HygR

Plasmid

#164134

Purpose

Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Long

Plasmid

#47395

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

intersectin long (ITSN1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAnc80L65

Plasmid

#68837

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

Ancestral AAV Capsid Anc80L65

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHEN2 F8-2

Plasmid

#186033

Purpose

Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

6His and Myc

Expression

Bacterial

Promoter

lac

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PB-3xCTCF-TetO

Plasmid

#213023

Purpose

piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

3xCTCF-TetO

Use

Mouse Targeting; Piggybase integration (transposo…

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CMV-Cas9-P2A-HygR

Plasmid

#164133

Purpose

Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRClover

Plasmid

#59151

Purpose

Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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ePB-PURO-FLAG-METTL3-APPA

Plasmid

#160253

Purpose

For stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposase

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

tight TRE promoter

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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V2R-GFP

Plasmid

#208910

Purpose

encodes the human vasopressin receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

AVPR2 (AVPR2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg-3xFlag

Plasmid

#223250

Purpose

Mammalian expression of C-terminally Flag-tagged R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV mKeima-LC3

Plasmid

#189006

Purpose

Fluorescent reporter for autophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

mKeima, LC3 (Map1lc3b Rat)

Use

Retroviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo-W42A

Plasmid

#179394

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo(W42A)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W42A

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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