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Showing: 11781 - 11800 of 65983 results

  4. pJYDNp

    Plasmid
    #162451
    Purpose
    Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA and myc
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162451-plasmid-map-sequence-id-321446

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  6. AICSDP-117:POLR2A-mEGFP

    Plasmid
    #164500
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Feb. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164500-plasmid-map-sequence-id-324900

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  9. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129025-plasmid-map-sequence-id-454365

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  12. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161815-plasmid-map-sequence-id-320654

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  13. pTRE3G-iRFP-B1-timestamp_A

    Plasmid
    #159924
    Purpose
    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.
    Depositor
    Fei Chen
    Publication
    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iRFP-Timestamp A
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    159924-plasmid-map-sequence-id-318472

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  17. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

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  18. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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Showing: 11781 - 11800 of 65983 results