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pCS2-HsProm1s1-mScarlet

Plasmid

#211330

Purpose

Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361)

Plasmid

#214857

Purpose

Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

jGCaMP8s

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13450: pAAV-AiE0140h-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN3450)

Plasmid

#214845

Purpose

Expression of iCre in striatal Pvalb neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pOP01-UTC-7.0

Plasmid

#216625

Purpose

NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

Depositor

Henrike Niederholtmeyer

Article

Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

Insert

T7p-5'g10-NanoLuc-3'TMV

Use

Synthetic Biology

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL

Plasmid

#218756

Purpose

Provides NOT intersectional (Cre NOT Flp) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL

Plasmid

#218755

Purpose

Provides NOT intersectional (Flp NOT Cre) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA3-Olônne

Plasmid

#214447

Purpose

Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SOX17-3'gRNA

Plasmid

#210465

Purpose

gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROS1-V5/HIS

Plasmid

#205609

Purpose

Expression of human ROS1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROS1 receptor tyrosine kinase (ROS1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-KDM2B-P2A-mCherry

Plasmid

#203852

Purpose

Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

KDM2B (KDM2B Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR4A3-P2A-mCherry

Plasmid

#203863

Purpose

Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A3 (NR4A3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pSyn_Mito-LOV-Turbo-V5

Plasmid

#199669

Purpose

expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

COX4I1 mito targeting sequence and V5

Promoter

Synapsin

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus reporter

Plasmid

#192458

Purpose

Fluorescent reporter for beta-TrCP activity in human cells

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CDC25B (CDC25B Human)

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338

Promoter

EF1alpha

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

Plasmid

#191368

Purpose

Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dHgm4Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

Plasmid

#191369

Purpose

Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

NLS-NLS-dPba3Cas13b-3xGFP-NLS

Tags

3xGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-177 pUC19-CLTA-N-mCherry

Plasmid

#186128

Purpose

knockin of mCherry to CLTA target

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CLTA (CLTA Human)

Use

CRISPR

Mutation

WT with mCherry sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_LMNA_G2

Plasmid

#178090

Purpose

Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-NQL005-SOX2-sgRNA

Plasmid

#175553

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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