We narrowed to 20,125 results for: Kin

1. pcDNA3 βarr1 S412A Flag

   Plasmid

   #42195

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   β-arrestin 1 S412A (Arrb1 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S412A

   Promoter

   CMV

   Available Since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-Spike-S-4P

   Plasmid

   #165094

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)
   

   Expression

   Bacterial

   Available Since

   March 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 Fyn deltaSH3

   Plasmid

   #16034

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Expression

   Mammalian

   Mutation

   Delta SH3: deletion of amino acids 76-141

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

5. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

   Plasmid

   #169126

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Expression

   Mammalian

   Mutation

   NAWLVH106-AAAAAA

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-hMeCP2-GFP-R270X

   Plasmid

   #48079

   Purpose

   Mammalian expression of GFP tagged MeCP2-R270X

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   expresses AT-Hook R270X

   Promoter

   CMV

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

   Plasmid

   #117384

   Purpose

   An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MERS-coV-NSP3-c001

   Plasmid

   #173085

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   D1109-D1275, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-G88P3-HA-hM3Dq

   Plasmid

   #213972

   Purpose

   AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Promoter

   G88P3

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-tetO-Meis1

    Plasmid

    #139824

    Purpose

    doxycycline-inducible expression of mouse Meis in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Meis1 (Meis1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-DIO-tdTomato-f

    Plasmid

    #127092

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Insert

    tdTomato-f
    

    Use

    AAV

    Tags

    farnesylation signal from c-Ha-Ras

    Expression

    Mammalian

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mito-yHS1-M7A

    Plasmid

    #159164

    Purpose

    Mitochondrial labile heme reporter

    Depositor

    Amit Reddi

    Article

    Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

    Insert

    mito-yHS1-M7A
    

    Tags

    COX4 pre-sequence

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of mito-yHS1…

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. p-mCherry-C1-iCoChR++

    Plasmid

    #98173

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iCoChR++ gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-CRY2

    Plasmid

    #140572

    Purpose

    For use in light-induced protein inactivation through the interaction with CIB1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFP-NGAT(A193T,N194Y)

    Plasmid

    #11388

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Changed Alanine 193 to Threonine, and Asparagine …

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-mGnptg

    Plasmid

    #78106

    Purpose

    Mammalian expression of mouse Gnptg with C-terminal His tag

    Depositor

    Thomas Braulke

    Article

    Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

    Insert

    Gnptg (Gnptg Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. mEmerald-Cx32-7

    Plasmid

    #54054

    Purpose

    Localization: Gap Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fort et al J Biol Chem. 2011 Jul 1;286(26):22875-85

    Insert

    Cx32 (GJB1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pSOMA-T679A-NLS

    Plasmid

    #118938

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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