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Showing: 11901 - 11920 of 65983 results

  7. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73209-plasmid-map-sequence-id-139734

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  8. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Publication
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  12. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    70703-plasmid-map-sequence-id-127765

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  16. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CYP450-CaNAR2
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    64732-plasmid-map-sequence-id-109351

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  17. pGEX 6P1 Cyclin A (555)

    Plasmid
    #61844
    Purpose
    bacterial expression of GST cyclin A
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    L2V
    Promoter
    tac
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 11901 - 11920 of 65983 results