We narrowed to 20,126 results for: Kin

1. pT7-gfap-sgRNA

   Plasmid

   #65566

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap sgRNA (gfap Zebrafish)
   

   Use

   In vitro rna transcription

   Promoter

   T7

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-GFP-TRIM28

   Plasmid

   #185010

   Purpose

   Encodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   TRIM28 sgRNA (Trim28 Mouse)
   

   Use

   CRISPR

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX302 PCSK5-V5 puro

   Plasmid

   #98389

   Purpose

   Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-ubb-cytoBirA-2a-mCherry

   Plasmid

   #79885

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

6. tet-pLKO shMKP5.v2 puro

   Plasmid

   #87793

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet-pLKO shMKP5.v1 puro

   Plasmid

   #87792

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-Stx3-mCitrine

   Plasmid

   #92428

   Purpose

   Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   Stx3 (Stx3 Rat)
   

   Tags

   FKBP (FK506 binding protein 12); allows for heter…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

   Plasmid

   #113952

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S and S212N su…

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Hygro-sgSIK3

    Plasmid

    #138699

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Y587E EphA2 pcDNA3

    Plasmid

    #102730

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Y587E (TAT > GAG)

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRM27

    Plasmid

    #163695

    Purpose

    ubb promoter driven expression of lck:mNeonGreen

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    lck:mNeonGreen (lck Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mNeonGreen

    Promoter

    ubb

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid

    #113956

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution…

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CIAR_pcDNA5/FRT/TO

    Plasmid

    #86502

    Purpose

    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CIAR (SOS1 Human)
    

    Use

    Frt

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV (tet operator)

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

20. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Tags

    DHFR and myc

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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